Molecular Neuropharmacology: Strategies and Methods

ISBN-13: 9781617373848 / Angielski / Miękka / 2010 / 271 str.

Arne Schousboe; Hans Brauner-Osborne; Hans Br Uner-Osborne

Opis

A powerful collection of readily reproducible cutting-edge techniques for characterizing the ligand or substrate binding of neurotransmitter receptors and transporters. The procedures cover interdisciplinary interactions for monoamine transporters, amino acid transporters, ionotropic receptors, metabotropic glutamate receptors, GABA receptors, and other G protein-coupled receptors. By illuminating how neurons in the central nervous communicate with other, these techniques can lead to the development of novel therapeutic strategies for neurological diseases.

Recenzje

"I would highly recommend this book to neuroscientists, pharmacologists, and medicinal chemists who are interested in ligand-macromolecular interactions. It provides descriptions of state-of-the-art tools needed to understand how CNS neurons communicate and perspectives regarding the development of novel therapeutic drugs for neurological diseases. Each chapter is well written and concise beginning with an appropriate introduction to the topic and ending with a brief summary, conclusion, or future perspective section. The references are selective and appropriate. Special features include cutting-edge techniques for using site directed mutagenesis and molecular modeling; novel insights into the functioning of transmitter receptors and transporters; and state-of-the-art discussions on the molecular pharmacology of GABA and glutamate receptors and transmitter transporters. "-Doody's Health Sciences Book Review Journal

"...provides a highly readable account...an excellent book." - ChemBioChem

Spis treści

Part I. Molecular Pharmacology of Glutamate Receptors Computational Studies of Ligand-Receptor Interactions in Ionotropic Glutamate Receptors Jeremy R. Greenwood and Tommy Liljefors Delineation of the Physiological Role of Kainate Receptors by Use of Subtype Selective Ligands Sari E. Lauri, Vernon R. J. Clarke, and Graham L. Collingridge Molecular Pharmacology of the Metabotropic Glutamate Receptors Anders A. Jensen Part II. Molecular Pharmacology of Gaba Receptors Functional Characterization of GABAA Receptor Ligands In Vitro: Correlation With In Vivo Activity Bjarke Ebert, Sally Anne Thompson, Signe Í Stórustovu, and Keith A. Wafford In Vivo Function of GABAA Receptor Subtypes Unraveled With Mutant Mice Jean-Marc Fritschy Characterization of the GABAA Receptor Recognition Site Through Ligand Design and Pharmacophore Modeling Bente Frøland, Anne T. Jørgensen, Tommy Liljefors, Martin Mortensen, and Povl Krogsgaard-Larsen The GABAB Receptor: From Cloning to Knockout Mice Bernhard Bettler and Hans Bräuner-Osborne Part III. Molecular Pharmacology of Transmitter Transporters Structure and Molecular Characterization of the Substrate-Binding Sites of the Dual-Function Glutamate Transporters Baruch I. Kanner, Michael P. Kavanaugh, and Lars Borre Allosteric Modulation of Glutamate Transporters Robert J. Vandenberg and Renae M. Ryan Characterization of the Substrate-Binding Site in GABA Transporters Alan Sarup, Orla Miller Larsson, and Arne Schousboe Insights From Endogenous and Engineered Zn21 Binding Sites in Monoamine Transporters Claus Juul Loland and Ulrik Gether A Structural Context for Studying Neurotransmitter Transporter Function Naomi R. Goldberg, Thijs Beuming, Harel Weinstein, and Jonathan A. Javitch Functional Mechanisms of GProtein-Coupled Receptors in a Structural Context Marta Filizola, Irache Visiers, Lucy Skrabanek, Fabien Campagne, and Harel Weinstein Index

Blurb

The ability to clone large numbers of receptors and transporters for neurotransmitters, and then to produce specific mutations of these proteins, has yielded significant understanding of their pharmacological and functional properties, with still deeper insight obtainable through the application of computational and medicinal chemistry. In Molecular Neuropharmacology: Strategies and Methods, leading neuroscience investigators combine advanced chemistry with molecular modeling to produce a powerful collection of cutting-edge techniques for characterizing the ligand or substrate binding of neurotransmitter receptors and transporters. The procedures cover interdisciplinary interactions for monoamine transporters, amino acid transporters, ionotropic receptors, metabotropic glutamate receptors, GABA receptors, and other G protein-coupled receptors. Information about x-ray crystallographic analyses is included whenever available.
State-of-the-art and highly practical, Molecular Neuropharmacology: Strategies and Methods provides detailed accounts of the tools needed to understand how CNS neurons communicate and promises the development of novel therapeutic agents and strategies for many neurological diseases.