Molecular Motors: Methods and Protocols

ISBN-13: 9781617377068 / Angielski / Miękka / 2010 / 252 str.

Ann O. Sperry

Opis

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.

Recenzje

From the reviews:

"Within this book's 16 chapters, readers will find a rich source of not only step-by-step protocols and useful lists of the chemicals, reagents, and equipment needed to perform them, but also useful troubleshooting tips and experimental notes that can assist in their successful execution. ... the book is a treasure of data, facts, protocols, and references that nearly every cell biologist and biochemist with an interest in molecular motors will cherish." (Tzvi Tzfira, The Quarterly Review of Biology, Vol. 83, 2008)

Spis treści

Contents Preface Contributors CH. 1 Equilibrium Binding of Proteins to F-Actin, Joseph M. Chalovich CH. 2 Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Cosedimentation and ATPase Assays, Anireddy S.N. Reddy CH. 3 In Vitro and In Vivo Analysis of Microtubule Destabilizing Kinesins, Jason Stumpff, Jeremy Cooper, Sarah Domnitz, Ayana Moore, Kathleen Rankin, Mike Wagenbach and Linda Wordeman CH. 4 Approaches to Kinesin-1 Phosphorylation, Gerardo Morfini, Gustavo Pigino, and Scott T. Brady CH. 5 Protein Modification to Probe Intra-dynein Interactions and In Vivo Redox State, Ken-ichi Wakabayashi, Miho Sakato and Stephen M. King CH. 6 Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains, Kevin W.-H. Lo and K. Kevin Pfister CH. 7 Identification of Motor Protein Cargo by Yeast 2-Hybrid And Affinity Approaches, Yuguo Zhang, Rong Wang, Holly Jefferson, and Ann O. Sperry CH. 8 In Situ Binding Assay to Measure Myosin-1c Interactions with Hair-Cell Proteins, Kelli R. Phillips and Janet L. Cyr CH. 9 Ultrastructural Analysis of Kinesin-related Motor Proteins During Spermatogenesis, Wan-Xi Yang CH. 10 In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting, John W. Murray and Allan W. Wolkoff CH. 11 Enrichment and Disassembly of Ectoplasmic Specializations in the Rat Testis, Julian A. Guttman, Kuljeet S.Vaid and A. Wayne Vogl CH. 12 Single-Molecule Observation of Rotation of F1-ATPase through Micro Beads, Takayuki Nishizaka, Kana Mizutani and Tomoko Masaike CH. 13 The use of FRET in the Analysis of Motor Protein Structure, Andre Kasprzak CH. 14 Structure Determination of the Motor Domain of Yeast Kinesin Kar3 by X-ray Crystallography, Hee-Won Park CH. 15 High Resolution Structural Analysis of the Kinesin-Microtubule Complex by Electron Cryo-Microscopy, Keiko Hirose and LindaA. Amos CH. 16 Chemical-Genetic Inhibition of Sensitized Mutant Unconventional Myosins, Ryan L. Karcher, D. William Provance, Jr., Peter G. Gillespie, and John A. Mercer