Molecular Microbiology: Diagnostic Principles and Practice

ISBN-13: 9781555819088 / Angielski / Twarda / 2016 / 835 str.

David H. Persing; Fred C. Tenover; Randall T. Hayden

Opis

Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice

• Presents the latest basic scientific theory underlying molecular diagnostics
• Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing
• Illustrates and summarizes key concepts and techniques with detailed figures and tables
• Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance
• Advises on the latest quality control and quality assurance measures
• Explores the increasing opportunities and capabilities of information technology

Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.

Spis treści

ContributorsPrefaceSection I: Novel and Emerging Technologies1 Nucleic Acid Amplification Methods Overview2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health4 Digital PCR and Its Potential Application to Microbiology5 Massively Parallel DNA Sequencing and Microbiology6 Next-Generation Sequencing7 Pathogen Discovery8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology9 Multiplex TechnologiesSection II: Metagenomics: Implications for Diagnostics10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice11 The Gastrointestinal Microbiome12 The Vaginal Microbiome13 Microbial Communities of the Male Urethra14 The Human Virome in Health and DiseaseSection III: Health Care-Associated Infections15 Molecular Detection of Staphylococcus aureus Colonization and Infection16 Molecular Diagnostics for Clostridium difficile17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms18 Detection of Vancomycin-Resistant EnterococciSection IV: Molecular Diagnostics and Public Health19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China22 Surveillance and Epidemiology of Norovirus Infections23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic DiseasesSection V: Syndromic Diagnostics24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections27 Molecular Diagnosis of Gastrointestinal Infections28 Diagnostic Approaches to Genitourinary Tract Infections29 Syndromic Diagnostic Approaches to Bone and Joint InfectionsSection VI: Virology30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 131 Molecular Detection and Characterization of Hepatitis C Virus32 Molecular Detection and Characterization of Hepatitis B Virus33 Molecular Detection of Human Papillomaviruses34 Molecular Diagnostics for Viral Infections in Transplant RecipientsSection VII: Fungi and Protozoa35 Molecular Detection and Identification of Fungal Pathogens36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance38 Molecular Detection of Gastrointestinal ParasitesSection VIII: Point-of-Care/Near-Care Diagnostics39 Molecular Diagnostics and the Changing Face of Point-of-Care40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings42 Rapid Point-of-Care Diagnosis of Malaria and Dengue InfectionSection IX: The Host and Host Response43 Implications of Pharmacogenetics for Antimicrobial Prescribing44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics45 Host Response in Human Immunodeficiency Virus Infection46 Biomarkers of Gastrointestinal Host Responses to Microbial InfectionsSection X: Information Technology47 Point-of-Care Medical Device Connectivity: Developing World Landscape48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South AfricaSection XI: Quality Assurance50 Molecular Method Verification51 Molecular Microbiology Test Quality Assurance and Monitoring52 Proficiency Testing and External Quality Assessment for Molecular Microbiology53 Practices of Sequencing Quality Assurance54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based ProtocolsSection XII: The Business of Diagnostics55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration56 Molecular Diagnostics and the Changing Legal LandscapeIndex