Molecular Methods in Plant Biology: A Comprehensive Book on Biotechnicological Aspects

ISBN-13: 9789386768810 / Angielski / Miękka / 2020 / 104 str.

Krishnananda Pralhad Ingle;Mangesh Pradip Moharil;Dipika Ashok Padole

Opis

This book integrates the experimental procedures and theoretical principles for undergraduate, postgraduate, academicians and researchers in the area of agriculture and life sciences. The experiments have been updated and extended to reflect developments in the respective fields. In-text worked examples are again used to enhance student’s understanding of each topic. The book is designed to provide students with the experience of how scientists use their knowledge to understand real-world science related issues that confront them in their professional lives. Experimental procedures and troubleshooting of data are emphasised throughout the book. The book integrates theory and practices to ensure students understand why and how each technique is used.

Spis treści

• 1. Isolation of Plant Genomic DNA
• 2. Isolation of Plasmid DNA
• 3. Polyacrylamide Gel Electrophoresis
• 4. Polymerase Chain Reaction (PCR) Amplification
• 5. RNA Isolation Using TRIzol Method
• 6. Expression Analysis Using Semi-Quantitative Real Time-PCR
• 7. Quantitative Expression Analysis by Real Time-PCR
• 8. Analysis of DNA Sequences by Southern Blotting and Hybridization
• 9. Analysis of DNA Sequences by Northern Blotting and Hybridization
• 10. Histochemical GUS Assay
• 11. Enzyme-Linked Immunosorbent Assay (ELISA)
• 12. DNA Gel Extraction Protocol
• 13. Competent Cell Preparation
• 14. Ligation, Transformation and Colony PCR
• 15. DNA Sequencing and Analysis
• Glossary
• Instruments Used in the Laboratory
• Appendix