Molecular Genetics of Asthma

ISBN-13: 9781493981076 / Angielski / Miękka / 2018 / 275 str.

Maria Isidoro Garcia

Spis treści

1. Applications of Molecular Genetics to the Study of Asthma

            Catalina S. Sanz Lozano, Virginia García-Solaesa, Ignacio Dávila González, and María Isidoro-García

 

2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach

            Belén García Berrocal, Asunción García-Sánchez, and María Isidoro-García

 

3. Introduction to the Gene Expression Analysis

            Ignacio San Segundo-Val and Catalina S. Sanz Lozano

 

4. Real Time PCR for Gene Expression Quantification in Asthma

            Ignacio San Segundo-Val, Virginia García-Solaesa, and Asunción García-Sánchez

 

5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma

            Ignacio San Segundo-Val and Catalina S. Sanz Lozano

 

6. Review of Methods to Study Gene Expression Regulation Applied to Asthma

            Asunción García-Sánchez and Fernando Marqués García

 

7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma

            Virginia García-Solaesa and Catalina S. Sanz Lozano

 

8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma

            Virginia García-Solaesa and Sara Ciria Abad

 

9. Chromatin Immunoprecipitation: Application to the Study of Asthma

            Asunción García-Sánchez and Fernándo Marqués-García

 

10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols

            Asunción García-Sánchez and Fernando Marqués-García

 

11. Protocols for Exosome Isolation and RNA Profiling

            Fernando Marqués-García and María Isidoro-García

 

12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell

            Elena Marcos-Vadillo and Asunción García-Sánchez

 

13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line

            Elena Marcos-Vadillo and Asunción García-Sánchez

 

14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line

            Elena Marcos-Vadillo and Asunción García-Sánchez

 

15. Review of Mouse Models Applied to the Study of Asthma

            Fernando Marqués-García and Elena Marcos-Vadillo

 

16. General and Specific Mouse Models for Asthma Research

            Fernando Marqués-García and Elena Marcos-Vadillo

 

17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma

            Fernando Marqués-García and Elena Marcos-Vadillo

 

18. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma

            Almudena Sánchez-Martín, Asunción García-Sánchez, and María Isidoro-García

Blurb

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.  Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.