1. Applications of Molecular Genetics to the Study of Asthma
Catalina S. Sanz Lozano, Virginia García-Solaesa, Ignacio Dávila González, and María Isidoro-García
2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach
Belén García Berrocal, Asunción García-Sánchez, and María Isidoro-García
3. Introduction to the Gene Expression Analysis
Ignacio San Segundo-Val and Catalina S. Sanz Lozano
4. Real Time PCR for Gene Expression Quantification in Asthma
Ignacio San Segundo-Val, Virginia García-Solaesa, and Asunción García-Sánchez
5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
Ignacio San Segundo-Val and Catalina S. Sanz Lozano
6. Review of Methods to Study Gene Expression Regulation Applied to Asthma
Asunción García-Sánchez and Fernando Marqués García
7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
Virginia García-Solaesa and Catalina S. Sanz Lozano
8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma
Virginia García-Solaesa and Sara Ciria Abad
9. Chromatin Immunoprecipitation: Application to the Study of Asthma
Asunción García-Sánchez and Fernándo Marqués-García
10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
Asunción García-Sánchez and Fernando Marqués-García
11. Protocols for Exosome Isolation and RNA Profiling
Fernando Marqués-García and María Isidoro-García
12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
Elena Marcos-Vadillo and Asunción García-Sánchez
13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Elena Marcos-Vadillo and Asunción García-Sánchez
14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
Elena Marcos-Vadillo and Asunción García-Sánchez
15. Review of Mouse Models Applied to the Study of Asthma
Fernando Marqués-García and Elena Marcos-Vadillo
16. General and Specific Mouse Models for Asthma Research
Fernando Marqués-García and Elena Marcos-Vadillo
17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma
Fernando Marqués-García and Elena Marcos-Vadillo
18. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma
Almudena Sánchez-Martín, Asunción García-Sánchez, and María Isidoro-García
Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.