Molecular Genetics and Breeding of Forest Trees

ISBN-13: 9781560229599 / Angielski / Miękka / 2004 / 436 str.

Sandeep Kumar; Matthias Fladung

Opis

This unique single source integrates tree transgenesis, functional genomics, and structural genomics to present, for the first time, a unified approach to research on the molecular biology of forest trees.

Spis treści

• About the Editors
• Contributors
• Preface
• Part I: FOREST TREE FUNCTIONAL GENOMICS
• Chapter 1. Functional Genomics in Forest Trees
• Introduction
• The Functional Genomics Tool Kit—Transcript Discovery and Profiling
• Functional Genomics in Action
• En Route to Functional Genomics in Forest Trees
• Conclusions
• Chapter 2. Expressed Sequence Tag Databases from Forestry Tree Species
• Introduction
• Forestry Tree EST Databases
• The Genesis Pine and Eucalyptus EST Projects
• Special Purpose Libraries
• Conclusion
• Chapter 3. Proteomics for Genetic and Physiological Studies in Forest Trees: Application in Maritime Pine
• Introduction
• Characterization of the Pine Proteome
• Genetic Variability of Qualitative and Quantitative Protein Variants
• Variability of Proteome Expression in Physiological Studies
• Conclusion
• Chapter 4. Exploring the Transcriptome of the Ectomycorrhizal Symbiosis
• Introduction
• The Ectomycorrhizal Symbiosis
• The Anatomy and Development of Ectomycorrhiza
• The Morphogenesis of Ectomycorrhiza
• Alteration in Gene Expression in Ectomycorrhiza
• The Transcriptome of Ectomycorrhizal Symbioses
• Outlook
• Part II: MOLECULAR BIOLOGY OF WOOD FORMATION
• Chapter 5. Genomics of Wood Formation
• Introduction
• Wood Biosynthesis
• Regulation of Wood Formation
• Arabidopsis As a Model for the Study of Wood Formation
• Genomics Approach to Wood Formation
• The Outlook
• Chapter 6. Molecular Genetics of Cellulose Biosynthesis in Trees
• Introduction
• Cellulose Heterogeneity in Trees
• Cellulose Production Under Mechanical Stress
• The Site of Cellulose Biosynthesis
• Current Knowledge of the General Process of Cellulose Synthesis in Plants
• Identification of CesA Genes in Plants
• Isolation of New CesA and CSLD Genes
• Future Perspectives
• Chapter 7. Tuning Lignin Metabolism Through Genetic Engineering in Trees
• Introduction
• Genetic Modification of PAL and C4H
• Genetic Modification of 4CL
• Genetic Modification of C3H
• Genetic Modification of CCoAOMT
• Genetic Modification of CCR
• Genetic Modification of F5H
• Genetic Modification of COMT
• Genetic Modification of CAD
• Genetic Modification of Peroxidases and Laccases
• Genetic Modification of Transcription Factors
• Outlook
• Chapter 8. In Vitro Systems for the Study of Wood Formation
• Introduction
• Cambial Activity
• Wood Formation
• The Formation of Tracheary Elements
• Heartwood Formation (Tylosis and Pit Aspiration)
• In Vitro Wood Formation Systems
• Future Perspectives
• Part III: FOREST TREE TRANSGENESIS
• Chapter 9. Genetic Modification in Conifer Forestry: State-of-the-Art and Future Potential—A Case Study with Pinus radiata
• Introduction
• Tissue Culture As a Tool for Molecular Research
• Genetic Engineering in Conifers
• Modification of Traits by Suppression of Gene Expression
• Analysis of Promoters Controlling Gene Expression in Conifers
• Tissue Culture Protocols for the Early Assessment of Gene Function
• Tools to Assess Gene Function at the Micro Level
• Conclusions
• Chapter 10. Transgenic Forest Trees for Insect Resistance
• Introduction
• Woody Plant Species Transgenic to Insect-Resistant Genes
• Insect-Resistant Genes
• Field Performance of Insect-Resistant Transgenic Trees
• Biosafety of Insect-Resistant Transgenic Plants
• Conclusion and Outlook
• Chapter 11. Modification of Flowering in Forest Trees
• Introduction
• Regulation of Flowering Time and Flower Development in Arabidopsis
• Genes Regulating Flower Development in Trees
• Prevention of Flowering
• Acceleration of Flowering
• Conclusions
• Chapter 12. Stability of Transgene Expression in Aspen
• Introduction
• rolC Gene, a Phenotypic Marker for the Stability of Transgene Expression
• Morphological Analysis of 35S-rolC Transgenic Aspen Plants
• Transgene Silencing
• Transgene Repeat Formation and Unstable Expression
• Transgene Integration and Unstable Expression
• Mechanisms of Transgene Integration and Repeat Formation
• How to Obtain Stably Expressing Transgenic Plants
• Future Perspectives
• Chapter 13. Asexual Production of Marker-Free Transgenic Aspen Using MAT Vector Systems
• Introduction
• Transformation Methods Using Agrobacterium
• Problems of Antibiotic Selection
• Principles of the MAT (Multi-Auto-Transformation) Vector System
• Two-Step Transformation of Tobacco Plants Using the GST-MAT Vector
• Transformation of Hybrid Aspen Using the GST-MAT Vector
• Current Techniques for Removing Selectable Markers and Their Problems
• Advantages of Selection Using the ipt Gene
• Transgene Stacking Using the ipt-MAT Vector
• Combination of the ipt and iaaM/H Genes
• Decrease in Environmental Impact Using Sterile Transgenic Trees
• Conclusion
• Part IV: GENOME MAPPING IN FOREST TREES
• Chapter 14. High-Density Linkage Maps in Conifer Species and Their Potential Application
• Introduction
• Basic Aspects for Constructing High-Density Maps in Conifer Species
• Application of High-Density Maps in Forest Species
• Conclusions and Perspectives
• Chapter 15. Microsatellites in Forest Tree Species: Characteristics, Identification, and Applications
• Introduction
• Microsatellites in the Nuclear Genome: Distribution and Variability
• From Genome to Populations: The Isolation of Microsatellites and the Development of Molecular Markers
• Microsatellites in Populations: SSRs As a Tool for the Screening of Diversity, Differentiation, and Evolution
• What is Different About Microsatellites of the Organelle Genomes?
• Conclusions
• Chapter 16. Genome Mapping in Populus
• Introduction
• Marker Systems
• Mapping Strategies
• Genetic Linkage Maps of the Populus Genome
• Comparison of Linkage Maps
• Perspectives
• Chapter 17. Genetic Mapping in Acacias
• Introduction
• Aspects of the Reproductive Biology of Acacias That Affect Genetic Mapping Strategies
• Linkage Mapping in Acacia mangium
• Applications of the Acacia Map
• Conclusions
• Index
• Reference Notes Included

O autorze

Sandeep Kumar, Mattias Fladung