Molecular Diagnostics in Cytopathology: A Practical Handbook for the Practicing Pathologist

ISBN-13: 9783319973968 / Angielski / Miękka / 2018 / 534 str.

Sinchita Roy-Chowdhuri; Paul Vanderlaan; John Stewart

Spis treści

Introduction:  Overview of Current Molecular Diagnostic Testing on Cytology Samples.- The Cytology Specimen and Preparations: Advantages and Limitations.- Sample Acquisition and Test Requisition.- Pre-Analytic Workflow and Specimen Evaluation.- DNA-Based Sequencing Assays.- RNA-Based Assays.- FISH Testing of Cytology Specimens: Pre-Analytic, Analytic and Post-Analytic Considerations.- Molecular Cytopathology Correlations: Interpretation of Molecular Diagnostic Results.- Human Papillomavirus (HPV) Testing of Head and Neck Cancers.- Human Papillomavirus (HPV) Testing on Cervical Cytology Specimens.- Molecular Diagnostics in Lung Cytology.- Molecular Diagnostics in Thyroid Cytology.- Molecular Diagnostics in Breast Cytology.- Molecular Diagnostics in Salivary Gland Cytology.- Molecular Diagnostics in Pancreatic and Biliary Cytology.- Fluorescence In Situ Hybridization (FISH) Testing in Urinary Tract Cytology.- Molecular Diagnostics in Hematological Malignancies.- Molecular Diagnostics in Bone and Soft Tissue Tumors.- Molecular Diagnostics in Pediatric Cytopathology.- Molecular Cytopathology: Final Thoughts and Future Directions.

O autorze

Sinchita Roy-Chowdhuri

The University of Texas MD Anderson Cancer Center

Houston, TX

USA

Paul A. VanderLaan 

Beth Israel Deaconess Medical Center

Harvard Medical School

Boston, MA

USA

John M. Stewart

The University of Texas MD Anderson Cancer Center

Houston, TX

USA

Gilda da Cunha Santos

Sunnybrook Health Science Center

Toronto, Ontario

Canada

Blurb

The main focus of this book is to provide an overview of the principles of molecular diagnostics in context of cytopathology specimens together with a basic understanding and working knowledge of the available technology, platforms, and clinical applications. The initial sections of the book summarize the pre-analytic aspects of molecular testing, including cytology specimen preparation and handling, specimen selection and evaluation, and workflow algorithms as well as the analytic considerations for a variety of nucleic acid and protein-based testing. The remaining section focuses on disease specific molecular testing for various organ-based applications. 

Molecular Diagnostics in Cytopathology: A Practical Handbook for the Practicing Pathologist serves as a useful resource for cytopathologists, surgical pathologists, molecular technologists, and all others dealing with the evolving field of molecular cytopathology.