Molecular Dermatology: Methods and Protocols

ISBN-13: 9781627032261 / Angielski / Twarda / 2013 / 460 str.

Cristina Has; Cassian Sitaru

Opis

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.

Spis treści

1. Molecular Dermatology Comes of Age

            Cristina Has and Cassian Sitaru

2. Molecular Diagnosis of Autoimmune Blistering Diseases

            Daisuke Tsuruta, Teruki Dainichi, Takahiro Hamada, Norito Ishii, and Takashi Hashimoto

3. Molecular Diagnosis of Genodermatoses

            Vesarat Wessagowit

4. Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections

            Ashraf Al-Amoudi and Achilleas S. Frangakis

5. 3D Visualization of Epidermal Langerhans Cells

            Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai

6. In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography

            Viviane Mumprecht and Michael Detmar

7. Optical Imaging of HPV Infection in a Murine Model

            Rhonda C. Kines, Hisataka Kobayashi, Peter L. Choyke, and Marcelino L. Bernardo

8. Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration

            Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider

9. Analysis of Cutaneous Somatic Mosaicism

            Anna M.G. Pasmooij and Marcel F. Jonkman

10. Global Proteome Analyses of SILAC Labeled Skin Cells

            Adrian Sprenger, Victoria Küttner, Leena Bruckner-Tuderman, and Jörn Dengjel

11. MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method

            Janosch Hildebrand, Adam Grundhoff, Stefan Gallinat, Horst Wenck, and Anja Knott

12. Cell Death in the Skin: How to Study Its Quality and Quantity?

            Roman Makarov, Peter Geserick, Maria Feoktistova, and Martin Leverkus

13. In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus

            Ken Ishii and Masayuki Amagai

14. Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo

            Mircea Teodor Chiriac

15. Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model

            Jean Albrengues, Guerrino Meneguzzi, and Cedric Gaggioli

16. Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues

            Alexander D. Boiko

17. Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors

            Benjamin Toh, Alessandra Nardin, Xilei Dai, Jo Keeble, Valerie Chew, and Jean-Pierre Abastado

18. Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice

            Michio Tomura and Kenji Kabashima

19. Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach

            Véronique J. Moulin

20. Bioengineered Skin Humanized Model of Psoriasis

            Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Río

21. Induction of Contact Hypersensitivity in the Mouse Model

            Stefan F. Martin

22. Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells

            Ganna Bilousova and Dennis R. Roop

23. RNAi-Mediated Gene Function Analysis in Skin

            Slobodan Beronja and Elaine Fuchs

 

24. Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies

            Wataru Nishie

25. Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII

            Alina Sesarman and Cassian Sitaru

26. Animal Model for Cutaneous Leishmaniasis

            Michael R. Fischer, Dominik John, Kordula Kautz-Neu, Anja Schermann, Kirsten Schwonberg, and Esther von Stebut

27. Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages

            Dominik John, Michael R. Fischer, and Esther von Stebut

28. Zebrafish as a Model System to Study Heritable Skin Diseases

            Qiaoli Li and Jouni Uitto

29. Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa

            Alexander Nyström, Leena Bruckner-Tuderman, and Johannes S. Kern

30. RNA Trans-Splicing for Genodermatoses

            Johann W. Bauer, Eva M. Murauer, Verena Wally, and Ulrich Koller

            Cristina Has and Cassian Sitaru

Blurb

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.