Molecular Cytogenetics: Protocols and Applications

ISBN-13: 9781617373008 / Angielski / Miękka / 2010 / 414 str.

Yao-Shan Fan

Opis

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis."

Recenzje

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics

Spis treści

Part I. Basic Concepts and Techniques Molecular Cytogenetics in Medicine: An Overview Yao-Shan Fan Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, and Kim A. Wilber Labeling Fluorescence In Situ Hybridization Probes for RNA Targets Ramesh Ramakrishnan and Larry E. Morrison Basic FISH Techniques and Troubleshooting Sue Van Stedum and Walter King Part II. Evolving Techniques and Applications Chromosome Microdissection Xin-Yuan Guan Primed In Situ Labeling Jørn Koch Spectral Karyotyping Jane Bayani and Jeremy A. Squire Multicolor FISH Syed M. Jalal and Mark E. Law Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization Brynn Levy and Kurt Hirschhorn Color Banding Vicki L. Hopwood, Christi J. Hada, and Armand B. Glassman Multicolor Fiber FISH Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap, Philip M. Kluin, and Ed Schuuring Multi-Telomere FISH Samantha J. L. Knight and Jonathan Flint Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements Laurence Colleaux, Solange Heuertz, Florence Molinari, and Marlene Rio Microarray CGH, Ben Beheshti, Paul C. Park, Ilan Braude, and Jeremy A. Squire Part III. Special Applications in Chromosomal Disorders Molecular Approaches for Delineating Marker Chromosomes Michael D. Graf and Stuart Schwartz Interphase FISH for Prenatal Diagnosis of Common Aneuploidies Baruch Feldman, Ayala Aviram-Goldring, and Mark I. Evans Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood, Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, and Joe LeighSimpson Preimplantation FISH Diagnosis of Aneuploidies Yury Verlinsky, Jeanine Cieslak, and Anver Kuliev Microdeletion Syndromes: Characteristics and Diagnosis Stuart Schwartz and Michael D. Graf Molecular Detection of Uniparental Disomy Wendy P. Robinson Molecular Cytogenetics in Reproductive Pathology Hélène Bruyère, Evica Rajcan-Separovic, and Dagmar K. Kalousek Part IV. Special Applications in Oncology Interphase FISH Studies of Chronic Myeloid Leukemia, Gordon W. Dewald Chromogenic In Situ Hybridization and FISH in Pathology Bae-Li Hsi, Sheng Xiao, and Jonathan A. Fletcher FISH Detection of HER2 Amplification in Breast Cancer Rosemary E. Mueller and Frances P. O'Malley Comparative Genomic Hybridization in Cancer Investigations Marjan M. Weiss, Mario A. J. A. Hermsen, Gerrit A. Meijer, and Paul J. van Diest Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells Yanming Zhang and Brigitte Schlegelberger BAC Resource for Molecular Cytogenetics Xiao-Ning Chen and Julie R. Korenberg Index

Blurb

With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations.
Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.