Molecular Biology of Erythropoiesis

ISBN-13: 9781461278979 / Angielski / Miękka / 2011 / 227 str.

Joao L. Ascensao; Joao L. Ascensao

Opis

This volume presents the proceedings of the Fourth Annual Symposium on the Molecular Biology of Hemopoiesis, held in Reno, Nevada, November 1 and 2, 1988. Its focus on erythropoiesis represents an attempt to cover a rapidly expanding field, which has gone from elegant studies of erythro- poietin physiology, to molecular biology, to clinical applications and again to physiology. The rapid development has been made possible by cloning of erythropoietin gene and the availability of recombinant hormone. The regulation of heme and its derivatives has also been aided by techniques of molecular biology; there is now a concerted effort to better understand how these enzymes contribute to proliferation, differentiation and maturation of the erythron. Globin gene derrangements have been targets of recent research in an attempt to correct the defect by genetic engineering. In the chapters of this book, several groups "expressed" their views on this subject. Finally, we analyze various regulators of erythropoiesis, both in vivo and in vitro. Dr. Richard Levere was a pioneer in many studies of heme metabolism and of erythropoiesis. He has been a generous supporter of research in this field and of our past meetings. It is only. fitting that this volume should be dedicated to him.

Spis treści

Biology of Erythropoietin.- Erythropoietin: From Mountain Top to Bedside.- Serum Erythropoietin and Hemoglobin Affinity for Oxygen in Patients Phlebotomized for Polycythemia Vera.- Erythropoietin and the Polycythemia of High-Altitude Dwellers.- Enhanced Effect of Increased Erythrocyte Production Rate on Plasma Erythropoietin Levels of Mice During Subsequent Exposure to Hypobaria.- Metabolism of Recombinant Human Erythropoietin in the Rat.- Regulation of Extrarenal Erythropoietin Production.- Structure and Role of Carbohydrate in Human Erythropoietin.- Cellular Localization of Erythropoietin Gene Transcription.- Regulation of the Erythropoietin Gene.- Heme Regulation and Erythropoiesis.- Studies of the Effect of Erythropoietin on Heme Synthesis.- Effects of Hemin on Erythropoiesis.- The Effect of Dimethyl Sulfoxide on Heme Synthesis and the Acute Phase Reaction in Human HepG2 Hepatoma Cells.- Cytochrome P450 Dependent Arachidonic Acid Metabolism in Hemopoietic Cells.- Regulation of Heme Biosynthesis in Chick Embryo Liver Cells.- Globin Gene Expression.- Long-Term Expression of the Human ß-Globin Gene After Retroviral Transfer into Pluripotent Hematopoietic Stem Cells of the Mouse.- Retroviral Gene Transfer in Mice: The Use of a Unique Packaging Line Improves Efficiency.- Expression of the Globin Genes and Hematopoiesis in Beta-Thalassemic Mice.- Butyric Acid Modulates Developmental Globin Gene Switching in Man and Sheep.- Erythropoiesis — Regulatory Aspects.- Tumor Necrosis Factor ? and the Anemia of Chronic Disease: Effects of Chronic Exposure to TNF on Erythropoiesis In Vivo.- Erythropoiesis in Cancer Patients Undergoing Immunotherapy.- Erythropoiesís in Murine Long Term Marrow Cultures.- Expression of Homeobox Genes in Human Erythroleukemia Cells.