Molecular Biology and Pathogenesis of Coronaviruses

ISBN-13: 9781461593751 / Angielski / Miękka / 2012 / 409 str.

P. J. Rottier; P. J. Rottier

Opis

The present volume contains the Proceedings of an EMBO Workshop organized in June 1983 by the Institute of Virology, Veterinary Faculty, State University of Utrecht, The Netherlands. Some 70 scientists from 11 countries followed the invitation to present and discuss their recent data on the structure, replication, genetics and pathogenesis of coronaviruses. It was the second international meeting on these viruses; the Workshop, which was held in Zeist near Utrecht followed the example of the Wuerzburg symposium of October 1980. At that time it became clear that coronaviruses are unique in many respects. Once a group of viruses that were defined merely on the basis of their characteristic peplomer morphology, Coronaviridae family members are known today - to be constructed from essentially three polypeptides - to use a "nested set" of 5-6 subgenomic mRNAs in the expression of their large, positive and single stranded RNA genome, - to generate these subgenomic RNAs through specific fusion of non- contiguous sequences, - to mature by budding from intracellular membranes, - to cause persistent infection with neurological involvement and sometimes immunopathological conditons. These and many other findings have been established only very recently. The articles collected in this book reveal and/or further detail these findings. Since these Proceedings contain the combined scientific presentations of representatives from virtually all laboratories engaged in the field, they provide a fairly comprehensive review of the state of the art in corona- virology.

Spis treści

Molecular Biology of Coronaviruses.- Overview.- Biochemistry of Coronaviruses 1983.- Viral Proteins and Maturation.- Organization of the IBV Genome.- Proteolytic Cleavage of the Peplomeric Glycoprotein E2 of MHV Yields Two 90K Subunits and Activates Cell Fusion.- Coronavirus Maturation.- In Vitro Assembly of the Murine Coronavirus Membrane Protein E1.- Characterization of Viral Proteins Synthesized in 229E Infected Cells and Effect(s) of Inhibition of Glycosylation and Glycoprotein Transport.- Defective Replication of Porcine Transmissible Gastroenteritis Virus in a Continuous Cell Line.- Structural Characterization of IBV Glycoproteins.- Use of Monoclonal Antibodies to Assess Antigenic Relationships of Avian Infectious Bronchitis Virus Serotypes in the United States.- Monoclonal Antibodies to the Three Classes of Mouse Hepatitis Virus Strain A59 Proteins.- Antigenic and Polypeptide Structure of Bovine Enteric Coronavirus as Defined by Monoclonal antibodies.- Plaque Assay, Polypeptide Composition and Immunochemistry of Feline Infectious Peritonitis Virus and Feline Enteric Coronavirus Isolates.- Assembly of 229E Virions in Human Embryonic Lung Fibroblasts and Effects of Inhibition of Glycosylation and Glycoprotein Transport on this Process.- Amphotericin Inhibits Coronavirus SD, SK and A59 Growth.- Electron Lucent Structures Induced by Coronaviruses.- Virus Specific RNAs: Structure and Replication.- Cloning and Sequencing the Nucleocapsid and E1 Genes of Coronavirus MHV-A59.- Nucleotide Sequencing of Mouse Hepatitis Virus Strain JHM Messenger RNA 7.- Transcription Strategy of Coronaviruses: Fusion of Non-Contiguous Sequences During mRNA Synthesis.- Studies on the Mechanism of RNA Synthesis of a Murine Coronavirus.- Glycoprotein E1 of MHV-A59: Structure of the O-linked Carbohydrates and Construction of Full Length Recombinant cDNA Clones.- DNA Sequencing Studies of Genomic cDNA Clones of Avian Infectious Bronchitis Virus.- The Genome of Transmissible Gastroenteritis Virus (TGEV).- Pathogenesis of Coronaviruses.- Overview.- Biology of Coronaviruses 1983.- MHV-A59 Pathogenesis in Mice.- Detection of MHV-A59 RNA by In Situ Hybridization.- Virological and Immunological Aspects of Coronavirus Induced Subacute Demyelinating Encephalomyelitis in Rats.- A One Year Study of Coronavirus SD Infection in Mice.- Immunosuppression with Cyclophosphamide Does Not Prevent Demyelination or Result in Uncontrolled Viral Replication in Coronavirus SD Infected Mice.- In Vivo and In Vitro Models of Demyelinating Diseases — VIII: Genetic, Immunologic and Cellular Influences on JHM Virus Infection of Rats.- Restricted Replication of a Temperature-Sensitive Mutant of MHV-A59 in Mouse Brain Astrocytes.- Replication of Murine Coronaviruses in Somatic Cell Hybrids Formed Between a Mouse Fibroblast Cell Line and either a Rat Schwannoma Line or a Rat Glioma Line.- Persistent In Vitro Infection with Mouse Hepatitis Virus 3.- Characterization of MHV-A59 Persistently Infected Cells.- Fine Specificity and Genetic Restriction of T Cell Clones Specific for Mouse Hepatitis Virus, Strain JHM.- The Immune Response to Mouse Hepatitis Virus: Genetic Variation in Antibody Response and Disease.- Pathogenic Differences Between Various Feline Coronavirus Isolates.- Expression of Feline Infectious Peritonitis (FIP) Coronavirus Antigens on the Surface of Feline Macrophage-Like Cells.- Role of Circulating Antibodies and Thymus-Dependent Lymphocytes in Production of Effusive Type Feline Infectious Peritonitis after Oral Infection.- Interactions of Porcine Enteric Corona-Virus TGEV with Macrophages and Lymphocytes.- Effect of Stomach and Gut Juices on Infectivity of Low and High Passaged Strains of T.G.E. Coronavirus: Properties of a Virus Mutant Resistant to Inactivation by Stomach Juice Obtained by Cycles of Survivor Selection in Tissue Culture.- Pathogenicity of Mouse Hepatitis Virus, MHV-2cc, from a Persistently Infected DBT Cell Line.- The Pathogenesis and Age Related Susceptibility of OC43 Virus in Mice.- Failure to Detect Coronavirus SK Antigen in Multiple Sclerosis Brain Tissue by Autoradiography.- Antiviral Action of Interferon in the Bovine Species: Study In Vitro and In Vivo.- The Effects of Mouse Hepatitis Virus Type 3 on the Microcirculation of the Liver in Inbred Strains of Mice.- Contributors.