Molecular Aspects of Hematologic Malignancies: Diagnostic Tools and Clinical Applications

ISBN-13: 9783642294662 / Angielski / Twarda / 2012 / 476 str.

Michal Witt; Malgorzata Dawidowska; Tomasz Szczepanski

Opis

This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors' own laboratories.

Recenzje

From the reviews:

"This book describes current diagnostic tools and clinical procedures used for hematological malignancies. ... The goal was to meet the needs of both clinical hematologists who treat patients with leukemia, lymphoma, and/or multiple myeloma and those who study these diseases in the laboratory. ... an excellent review of molecular biology for specific hematological malignancies and disorders. It also covers important molecular testing techniques that are commonly used in the diagnosis, management, and surveillance of these malignancies. ... a valuable addition to any hematologist's library." (John Patrick Galvin, Doody's Review Service, October, 2012)

Spis treści

Part I  MOLECULAR BIOLOGY OF SELECTED HEMATO-ONCOLOGIC DISEASES

 

1.        Molecular biology of acute lymphoblastic leukemia

Małgorzata Dawidowska, Monika  D. Kraszewska, Katarzyna Derwich, and Tomasz Szczepański

2.        Genetic mechanisms and molecular markers of neoplastic transformation in acute myeloid leukemia

Agata A. Filip, Marta Libura, Sebastian Giebel, and Olga Haus

3.        Molecular pathogenesis of aggressive B-cell lymphomas

Przemysław Juszczyński and Krzysztof Warzocha

4.        Molecular biology and classification of multiple myeloma

Anna Dmoszyńska and Norbert Grząśko

5.        Chronic lymphocytic leukemia

Anton W. Langerak and Yorick Sandberg

6.        The molecular biology of chronic myeloid leukemia

Tomasz Sacha, Kajetana Foryciarz, and Aleksander B. Skotnicki

7.        Molecular biology of myelodysplastic syndromes

Francesc Solé

8.        Myeloproliferative neoplasms

Andrzej Hellmann, Maria Bieniaszewska, Witold Prejzner, and Aleksandra Leszczyńska

 

Part II  DIAGNOSTICS AND MONITORING OF THERAPY OF HEMATO-ONCOLOGIC DISEASES

 

9.        Flow cytometric immunophenotyping as diagnostic tool of hematopoietic malignancies

Łukasz Sędek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena, Julia Almeida, Alberto Orfao, and Tomasz Szczepański

10.     Cytogenetics in hematology

Olga Haus, Anna Poluha, and Katarzyna Skonieczka

11.     Monitoring of minimal residual disease in acute lymphoblastic leukemia

Tomasz Szczepański, Małgorzata Dawidowska, and Katarzyna Derwich

12.     Gene expression profiling in hematologic malignancies

Przemysław Juszczyński, Bjoern Chapuy, Małgorzata Szczepaniak, and Krzysztof Warzocha

 

Part III  MOLECULAR PROCEDURES INVOLVED IN HEMATOPOIETIC STEM CELL TRANSPLANTATION

 

13.     Allogeneic transplantation of hematopoietic stem cells

Jacek Wachowiak

14.     Genetic basis of donor-recipient matching in allogeneic transplantation
of hematopoietic stem cells

Jacek Nowak and Jacek Wachowiak

15.     Chimerism following allogeneic transplantation of hematopoietic stem cells

Małgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak, and

Michał Witt

16.     Peritransplantation monitoring of minimal residual disease in acute lymphoblastic leukemia

Małgorzata Dawidowska, Katarzyna Derwich, and Tomasz Szczepański

17.     Biobanks of cellular material

Wiesław Wiktor-Jedrzejczak and Elzbieta Urbanowska

 

Part IV  MOLECULAR DIAGNOSTIC PROTOCOLS

 

18.     Biobanking of cellular material

Anna Poluha and Elżbieta Urbanowska

19.     Isolation of mononuclear cells from human blood and bone marrow by density gradient centrifugation

Małgorzata Dawidowska

20.     Molecular techniques commonly used in hematooncolology

Monika D. Kraszewska and Ewa Ziętkiewicz

21.     Genetic methods of HLA typing

Jacek Nowak, Renata Mika-Witkowska, and Elżbieta Graczyk-Pol

22.     Post-transplant chimerism analysis through STR-PCR and RQ-PCR

Małgorzata Dawidowska, Katarzyna Guz,  Agnieszka Orzińska, Justyna Smolarczyk-Wodzyńska, Monika D. Kraszewska, and Michał Witt

23.     Analysis of minimal residual disease with the use of rearrangements of Ig/TCR genes

through RQ-PCR

M. Dawidowska, Vincent H. J. van der Velden, Michał Witt, and Tomasz Szczepański

24.     Molecular diagnostics of acute myeloblastic leukemia

Marta Libura, Agata A. Filip, and  Olga Haus

25.     Assessment of the presence and the level of BCR-ABL fusion gene expression and mutational status in ABL kinase domain.

Sylwia Czekalska, Magdalena Zawada, and Izabela Florek

26.     Studies of rearrangements and somatic hypermutation of IGHV genes in chronic lymphocytic leukemia

Anton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi, Kostas Stamatopoulos, and Frederic Davi  for the ERIC-IgCLL group

27.     Molecular biology methods in the diagnosis of multiple myeloma

Anna Dmoszyńska and Sylwia Chocholska

28.     Molecular methods in myeloproliferative neoplasms

Aleksandra Leszczyńska, Witold Prejzner, Maria Bieniaszewska, and Andrzej Hellmann

29.     Informed consent for participation in research project

Małgorzata Dawidowska and Michał Witt