Molecular Analyses

ISBN-13: 9780367903718 / Angielski / Twarda / 2022 / 336 str.

Opis

Methods for extraction, manipulation, and characterization of nucleic acids, peptides, and proteins have been rapidly developed. Extraction from a variety of tissues and samples have become routine and automated. Next generation sequencing (NGS) methods have been developed. This book focuses on medically-focused methods.

Spis treści

Preface. List of Contributors. Nucleic Acid Extraction from Diverse Samples. DNA Extraction from Mummified Tissues. Commercial DNA Extraction Kits. Automated Nucleic Acid Extraction. Forensic DNA Samples: Collection and Handling. DNA Preservation. RNA Storage. Disposable Electrochemical DNA Biosensors. Handheld Nucleic Acid Analyzer. DNA-Binding Fluorophores. Fluorescence Resonance Energy Transfer (FRET). LightUp® Probes. Invader® Assay. Laser-Capture Microdissection. Helper-Dependent Adenoviral Vectors. Retroviral Vectors. Liposomal Nonviral Delivery Vehicles. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing. Automated DNA Sequencing. DNA Sequencing Methods. Differential Sequencing by Mass Spectrometry. Brief Guide to Conducting Biological Database Searches. Accessing Genomic Databases. Phylogenetics, Comparative Genomics, and Phylogenomics. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences. ESTAnnotator: A Tool for High-Throughput EST Annotation. The Basics of Omics. Ribotyping. Forensic DNA Typing: Y Chromosome. Forensic Identification: An Overview on Molecular Diagnostic Technology. Denaturing Gradient Gel Electrophoresis (DGGE). Heteroduplex Analysis (HA). NEBcutter: A Program to Cleave DNA with Restriction Enzymes. Differential Display (DD) Analysis. Protein Truncation Test (PTT). Index.

O autorze

Scott Orland Rogers is a professor of molecular biology and evolution at Bowling Green State University, Bowling Green, Ohio. He received his PhD in plant molecular biology from the University of Washington, Seattle. He was an assistant professor and associate professor at the State University of New York College of Environmental Science and Forestry before moving to BGSU. He has taught courses in biology, botany, cell physiology, molecular biology, molecular genetics, bioinformatics, and molecular evolution. Research in his lab includes studies of microbes and nucleic acids preserved in ice, life in extreme environments, group I introns, molecular microbial phylogenetics, microbial metagenomics/metatranscriptomics, ancient DNA, and plant development.