Modification of Ti Plasmid to Construction dTi Vector for Gene Cloning

ISBN-13: 9786134981644 / Angielski / Miękka / 2018 / 112 str.

Hisham Faiadh

Opis

The extracted DNA of Agrobacterium tumefaciens isolates were subjected to PCR for amplifying 16S rRNA and for amplifying T-DNA fragment then subjected to gel electrophoresis. The individual band of the 16S rRNA gene was characterized by 1479bp and of the T-DNA fragment by 1200bp. The products were comparison with the standard molecular DNA ladder (1200 and 1500bp). The purified beta-lactamase gene was cut from PGLO plasmid ligated by use T4DNA ligase enzyme with the Ti plasmid which disarmed T-DNA by using the same restriction enzyme. The result was indicated by using the E.coli K12 for carries the Ti plasmid vector which contain a beta-Lactamase gene when put the antibiotic ampicillin in different consternation into the LB, MHA plate only the colonies which that have picked up exogenous DNA (dTi plasmid DNA ) can grow that is mean it become resistance to ampicillin by using dTi vector.

O autorze

Hisham Faiadh is a PhD student in Sheffield Hallam University. MERI and BMRC, 2015-Now. MSc. in Genetic Engineering, Basrah University, Science College, Biology Dep. 2008-2010. Baghdad University, Genetic Engineering and Biotechnology Institute for post graduate studies. Higher Dploma in Genetic Engineering 2004-2005. Bachelor in Vm & S 1998-2003.