Modern Methods in Protein Chemistry. Volume 3

ISBN-13: 9783112328019 / Angielski / Twarda / 1988 / 394 str.

Harald Tschesche

Spis treści

Frontmatter -- PREFACE -- CONTENTS -- STRATEGIES FOR THE LOCALIZATION AND SYNTHESIS OF PROTEIN BINDING SITES -- CONTINUOUS FLOW PEPTIDE SYNTHESIS -- SPECIFICITIES OF AUTOANTIBODIES TO SMALL NUCLEAR RIBONUCLEOPROTEINS (U snRNPs) IN SERA FROM PATIENTS WITH CONNECTIVE TISSUE DISEASES -- NEW ULTRASENSITIVE DETECTION SYSTEMS fOR BIOLUMINESCENCEENHANCED ENZYME IMMUNOASSAYS -- PRACTICAL CONSIDERATIONS IN BIOLUMINESCENCE-ENHANCED ENZYME IMMUNOASSAYS ILLUSTRATED BY A NEUTRAL PROTEINASE INHIBITOR OF PORCINE LEUKOCYTES -- A NEW, VERY SENSITIVE, BIOLUMINESCENCE-ENHANCED DETECTON SYSTEM fOR PROTEIN BLOTTING (WESTERN BLOT) -- THE USE OF COLLOIDAL METAL PARTICLES IN PROTEIN BLOTTING -- AMINO-ACID SEQUENCE ANALYSIS OF PROTEINS SEPARATED BY ONE- AND TWO-DIMENSIONAL GEL ELECTROPHORESIS AND ELECTROBLOTTED ON POLYBASE-COATED GLASSFIBER SHEETS -- PHOTOAFFINITY LABELING AND LOCALIZATION BY MICROSEQUENCING OF AN ION CHANNEL PROTEIN -- MICROCHARACTERIZATION OF PHOSPHOSERINE CONTAINING PROTEINSLOCALIZATION OF THE AUTOPHOSPHORYLATION SITES OF SKELETAL MUSCLE PHOSPORYLASE KINASE -- NEW MODULES FOR THE CONSTRUCTION OF A GASPHASE-SEQUENCER -- A NOVEL SOLID SUPPORT FOR GAS-LIQUID MICROSEQUENCING OF PROTEINS AND PEPTIDES - DERIVATIZED POROUS GLASS REPLACES POLYBRENE-COATED GLASFIBER FILTER -- NEW AUTOMATED AMINO ACID ANALYSIS BY HPLC PRECOLUMNS DERIVATISATION WITH FLUORENYLMETHYLOXYCARBONYLCHLORIDE -- HICROCALORIMETRY OF PROTEIN - LIGAND INTERACTION AND PROTEIN UNFOLDING -- APPLICATION OF BIFUNCTIONAL REAGENTS FOR TOPOLOGICAL INVESTIGATION -- TOWARDS THE IDENTIFICATION OF THE NUCLEOTIDE-BINDING SITE OF TUBULIN -- CRYSTALLOGRAPHIC AND IMAGE RECONSTRUCTION STUDIES ON RIBOSOMES -- Cell Culture Techniques for Testing of Biologically Active Peptides and Drugs: Clonogenic Assays Using Agar-Containing Glass Capillaries -- ELECTROTRANSFORMATION OF CULTURE CELLS -- AUTHOR INDEX -- Subject Index