Modern Analytical Ultracentrifugation: Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems

ISBN-13: 9781468468304 / Angielski / Miękka / 2012 / 354 str.

Todd M. Schuster; Thomas M. Laue; Todd M. Schuster

Opis

There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation."

Spis treści

I: Sedimentation Equilibrium.- Notes on the Derivation of Sedimentation Equilibrium Equations.- Association of REI Immunoglobulin Light Chain VL Domains: The Functional Linearity of Parameters in Equilibrium Analytical Ultracentrifuge Models for Self-Associating Systems.- Comments on the Analysis of Sedimentation Equilibrium Experiments.- The Omega Analysis and the Characterization of Solute Self- Association by Sedimentation Equilibrium.- Conservation of Signal: A New Algorithm for the Elimination of the Reference Concentration as an Independently Variable Parameter in the Analysis of Sedimentation Equilibrium.- Analysis of Protein—Nucleic Acid and Protein—Protein Interactions Using Multi-Wavelength Scans from the XL-A Analytical Ultracentrifuge.- II: Sedimentation Velocity.- Sedimentation Boundary Analysis of Interacting Systems: Use of the Apparent Sedimentation Coefficient Distribution Function.- Studies of Macromolecular Interaction by Sedimentation Velocity.- Measuring Sedimentation, Diffusion, and Molecular Weights of Small Molecules by Direct Fitting of Sedimentation Velocity Concentration Profiles.- Computer Simulation of the Sedimentation of Ligand-Mediated and Kinetically Controlled Macromolecular Interactions.- Refining Hydrodynamic Shapes of Proteins: The Combination of Data from Analytical Ultracentrifugation and Time-Resolved Fluorescence Anisotropy Decay.- III: Acquisition and Data Reduction.- On Line Data Acquisition for the Rayleigh Interference Optical System of the Analytical Ultracentrifuge.- Extensions to Commercial Graphics Packages for Customization of Analysis of Analytical Ultracentrifuge Data.- A Graphical Method for Determining the Ideality of a Sedimenting Boundary.- IV: Some Specific Examples.- Analytical Ultracentrifugation and Its Use in Biotechnology.- Applications of Analytical Ultracentrifugation in Structure-Based Drug Design.- The Sedimentation Equilibrium Analysis of Polysaccharides and Mucins: A Guided Tour of Problem Solving for Difficult Heterogeneous Systems.