Mitosis: Methods and Protocols

ISBN-13: 9781071619032 / Angielski / Twarda / 2021

Edward H. Hinchcliffe

Spis treści

1. Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

            Debadrita Pal, Florencia Visconti, Silvia P. Sepúlveda-Ramírez, S. Zachary Swartz, and Charles B. Shuster

 

2. Methods for Investigating Cell Division Mechanisms in C. elegans

            Ian D. Wolff, Nikita S. Divekar, and Sarah M. Wignall

 

3. Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes

            Brandt Warecki, Ian Bast, and William Sullivan

 

4. Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies

            Sidney L. Shaw, Mathew Siebe, and Timothy Cioffi

 

5. Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells

            John R. Daum, Casey O. DuBose, Sushama Sivakumar, and Gary J. Gorbsky

 

6. Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

            Katie B. Shannon

 

7. Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin

            Sally P. Wheatley

 

8. Using Microinjection of Mammalian Cultured Cells to Study Cell Division

            Charles Day, Alyssa Langfald, and Edward H. Hinchcliffe

 

9. In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments during Mitosis

            Sana Afreen, Amit Rahi, Adriana G. Landeros, Manas Chakraborty, Richard J. McKenney, and Dileep Varma

 

10. Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity

            Alex F. Thompson, Sarah Vandal, and Jason Stumpff

 

11. Chemical Biology of Mitotic Spindle Assembly Motors

            April Solon and Ryoma Ohi

 

12. Deploying Kinase Inhibitors to Study Pediatric Gliomas

            Benjamin T. Himes, Liang Zhang, and David J. Daniels

 

13. Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells

            Ana Maria G. Dumitru and Duane A. Compton

 

14. Degron Tagging Using mAID and SMASh Tags in RPE-1 Cells

            Adrijana Crncec and Helfrid Hochegger

 

15. Centromere Tension Measurement in Budding Yeast Mitosis

            Soumya Mukherjee and Melissa K. Gardner

 

16. Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo

            Yunyan He, David Adalsteinsson, Benjamin Walker, Josh Lawrimore, M. Gregory Forest, and Kerry Bloom

 

17. Using FLIM-FRET for Characterizing Spatial Interactions in the Spindle

            Stephanie C. Ems-McClung and Claire E. Walczak

 

18. Imaging Mitosis with Lattice LightSheet

            Andrew Barazia and Colin Monks

Blurb

This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time.