Mitochondrial Regulation: Methods and Protocols

ISBN-13: 9781493918744 / Angielski / Twarda / 2014 / 201 str.

Carlos M. Palmeira; Anabela Pinto Rolo; Anabela P. Rolo

Opis

In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

Spis treści

1. Isolation of Crud Mitochondrial Fraction from Cells

Mariusz R. Wieckowski and Lech Wojtczak

 

2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein – Analyses of MicroRNA and Protein Expressions

Pedro M. Borralho, Clifford J. Steer, and Cecília M. P. Rodrigues

 

3. PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species

Rooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, Mayer GD, Greenamyre JT, and Meyer JN

 

4. NAD⁺ Content and its Role in Mitochondria

Wei Li and Anthony A. Sauve

 

5. Measuring PGC-1α and its Acetylation Status in Mouse Primary Myotubes

Ana P. Gomes and David A. Sinclair

 

6. Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes

Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial

 

7. Determination Of Oxidative Phosphorylation Complexes Activities

João S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo

 

8. Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects

Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski

 

9. A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies

Fabrice Rappaport

 

10. BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial Function

Elena Silvestri, Assunta Lombardi, Federica Cioffi , and Fernando Goglia

 

11. Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus Mitochondria

Kieran J. Clarke, Audrey M. Carroll, Gemma O’Brien & Richard K. Porter

 

12. Exploring Liver Mitochondrial Function By ¹³c-Stable Isotope Breath Tests. Implications In Clinical Biochemistry

Ignazio Grattagliano, Leonilde Bonfrate, Michele Lorusso, Luigi Castorani, Ornella de Bari, and Piero Portincasa

 

13. Following Mitochondria Dynamism: Confocal Analysis of The Organelle Morphology

F. R. Mariotti, M. Corrado, and S. Campello

14. Analysis of Pro-apoptotic Protein Trafficking to and From Mitochondria

Ignacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa Serafim, Vilma Sardao, and Paulo J. Oliveira

 

15. Mitophagy and Mitochondrial Balance

Simone Patergnani and Paolo Pinton