Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function

ISBN-13: 9781493922871 / Angielski / Twarda / 2015 / 447 str.

Volkmar Weissig; Marvin Edeas

Opis

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

Spis treści

1. DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: Preparation, Characterization, and Use

            Volkmar Weissig

 2. Predicting Mitochondrial Targeting by Small Molecule Xenobiotics Within Living Cells Using QSAR Models

            Richard W. Horobin

 3. Targeting Mitochondria with Small Molecules: The Preparation of MitoB and MitoP as Exomarkers of Mitochondrial Hydrogen Peroxide

            Andrew G. Cairns, Stephen J. McQuaker, Michael P. Murphy, and Richard C. Hartley

 4. Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes

            Parul Benein, Mohammed Almuteri, Ahmed Mehanna, and Gerard G.M. D’Souza

 5. Synthesis and Evaluation of ¹⁸F-Labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging

            Dong-Yeon Kim and Jung-Joon Min

 6. Bridging the Gap Between Nature and Antioxidant Setbacks: Delivering Caffeic Acid to Mitochondria

            José Teixeira, Pedro Soares, Sofia Benfeito, Michael P Murphy, Paulo J. Oliveira, and Fernanda Borges

 7. Mitochondriotropic Nano-Emulsified Genistein-Loaded Vehicles for Cancer Therapy

            Jimmy Pham, Oliver Grundmann, and Tamer Elbayoumi

 8. Formulation and Optimization of Mitochondria-Targeted Polymeric Nanoparticles

            Sean Marrache, Rakesh K. Pathak, and Shanta Dhar

 9. Ex Vivo Generation of Functional Immune Cells by Mitochondria-Targeted Photosensitization of Cancer Cells

            Sean Marrache, Smanla Tundup, Donald A. Harn, and Shanta Dhar

 10. Targeting the Mitochondrial Genome Via a Dual Function MITO-Porter: Evaluation of mtDNA-Levels and Mitochondrial Function

            Yuma Yamada and Hideyoshi Harashima

 11. Mitochondrial, Acidic, and Cytosolic pHs Determination by ³¹P NMR Spectroscopy: Design of New Sensitive Targeted pH Probes

            Marcel Culcasi, Sophie Thétiot-Laurent, Ariane Atteia, and Sylvia Pietri

 12. Methodology for Use of Mitochondria-Targeted Cations in the Field of Oxidative Stress-Related Research

            Mikhail Y. Vyssokikh, Yury N. Antonenko, Konstantin G. Lyamzaev, Tatyana I. Rokitskaya, and Vladimir P. Skulachev

 13. Synthesis and Testing of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin

            Lucia Biasutto, Andrea Mattarei, and Cristina Paradisi

 14. Evaluation of Respiration of Mitochondria in Cancer Cells Exposed to Mitochondria-Targeted Agents

            Katarina Kluckova, Lan-Feng Dong, Martina Bajzikova, Jakub Rohlena, and Jiri Neuzil

 15. The Effect of Mitochondrially Targeted Anti-Cancer Agents on Mitochondrial (Super)Complexes

Magdalena Vondrusova, Ayanachew Bezawork-Geleta, Karishma Sachaphibulkij, Jaroslav Truksa, and Jiri Neuzil

 16. Mitochondrial Targeting of Recombinant RNA

            Ilya Dovydenko, Anne-Marie Heckel, Yann Tonin, Ali Gowher, Alya Venyaminova, Ivan Tarassov, and Nina Entelis

 17. Mitochondrial Targeting of Catalytic RNAs

            Daria Mileshina, Adnan Khan Niazi, Eliza Wyszko, Maciej Szymanski, Romain Val, Clarisse Valentin, Jan Barciszewski, and André Dietrich

 18. Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease

            David A. Dunn and Carl A. Pinkert

 19. Analysis of Pollutant-Induced Changes in Mitochondrial DNA Methylation

            Hyang-Min Byun and Timothy M. Barrow

 20. ETS and DEAS Studies of the Reduction of Xenobiotics in Mitochondrial Intermembrane Space

            Stanislav A. Pshenichnyuk and Alberto Modelli

 21. Analysis of Mitochondrial Morphology and Function Under Conditions of Mitofusin 2 Deficiency

            Juan Pablo Muñoz and Antonio Zorzano

 22. Yeast as a Tool to Study Mitochondrial Retrograde Pathway En Route to Cell Stress Response

            Maša Ždralević, Nicoletta Guaragnella and Sergio Giannattasio

 23. Cell Energy Budget Platform for Assessment of Cell Metabolism

            Dmitri B. Papkovsky and Alexander V. Zhdanov

 24. Induced Pluripotent Stem Cells (iPSCs) for Modeling Mitochondrial DNA Disorders

            Alessandro Prigione

 25. Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice

            Thomas Kolbe, Ralf Steinborn, and Joerg P. Burgstaller

 26. In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans

            Reuben L. Smith, Winnok H. De Vos, Richard de Boer, Erik M.M. Manders, and Hans van der Spek

 27. Mitochondrial DNA as a Biosensor of UV Exposure in Human Skin

            Amy Bowman and Mark A. Birch-Machin

 28. Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation

            Matthias Hecker, Natascha Sommer, and Konstantin Mayer

 29. Atomic Force Microscopy-Based Shape Analysis of Heart Mitochondria

            Gi-Ja Lee and Hun-Kuk Park

 30. Enzymatic Assays for Probing Mitochondrial Apoptosis

            Zhenyu Wang, Claire Nicolas, Rodolphe Fischmeister, and Catherine Brenner

 31. A Cybrid Cell Model for the Assessment of the Link Between Mitochondrial Deficits and Sporadic Parkinson’s Disease

            Daniela M. Arduíno, A. Raquel Esteves, Russell H. Swerdlow, and Sandra M. Cardoso

 32. Analysis of Mitochondrial Network by Imaging: Proof of Technique in Schizophrenia

            Dorit Ben-Shachar, Edith Suss-Toby, and Odile Robicsek

Blurb

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Methods and Protocols, Volume II: Manipulating Mitochondrial Function describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine: Methods and Protocols provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.