Mitochondrial Medicine: Volume 2: Assessing Mitochondria

ISBN-13: 9781071612682 / Angielski / Miękka / 2022

Weissig, Volkmar

Opis

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Spis treści

1. Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow

Naig Gueguen, Guy Lenaers, Pascal Reynier, Volkmar Weissig, and Marvin Edeas

 

2. Preparation of ‘Functional’ Mitochondria – A Challenging Business

Stefan Lehr, Sonja Hartwig, and Jorg Kotzka

 

3. Isolation and Quality Control of Functional Mitochondria

Sonja Hartwig, Jorg Kotzka, and Stefan Lehr

 

4. Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient

Jacob L. Léger, Nicolas Pichaud, and Luc H. Boudreau

 

5. Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues

Alessandro Gaviraghi, Yan Aveiro, Stephanie S. Carvalho, Rodiesley S. Rosa, Matheus P. Oliveira, and Marcus F. Oliveira

 

6. Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems

Nicoletta Guaragnella, Maša Ždralević,  Zdena Palková, and Sergio Giannattasio

7. Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes

Gisela Beutner and George A. Porter, Jr.

 

8. Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging

 Lori M. Buhlman, Petros P. Keoseyan, Kathryn Houlihan, and Amber N. Juba

 

9. Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays

Flavia Radogna, Déborah Gérard, Mario Dicato, and Marc Diederich

 

10. Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues

Juan C. Landoni, Liya Wang, and Anu Suomalainen

 

11. Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria

Camille Colin, Emmanuel Suraniti, Emma Abell, Audrey Sémont, Neso Sojic, Philippe Diolez, and Stéphane Arbault

 

12. Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence

Ann-Katrin Hopp and Michael O. Hottiger

 

13. Assessment of Mitochondrial Ca²⁺ Uptake

András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier, and Markus Waldeck-Weiermair

 

14. Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices

Andrey Y. Vinokurov, Viktor V. Dremin, Gennadii A. Piavchenko, Olga A. Stelmashchuk, Plamena R. Angelova, and Andrey Y. Abramov

 

15. Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis

Hsiu-Han Fan, Tsung-Lin Tsai, Ivan L. Dzhagalov, and Chia-Lin Hsu

 

16. Analysis of Mitochondrial Dysfunction during Cell Death

Vladimir Gogvadze and Boris Zhivotovsky

 

17. Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes

Sergiy M. Nadtochiy, Megan Ngai, and Paul S. Brookes

 

18. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane

Piotr Bednarczyk, Rafał P. Kampa, Shur Kucman, Aleksandra Sęk, Agnieszka Walewska, and Piotr Koprowski

 

19. Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway

Ana S. Almeida, Cláudia Figueiredo-Pereira, and Helena L.A. Vieira

 

20. 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction

Shima Mehrvar, Amadou K. S. Camara, and Mahsa Ranji

 

21. Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology

Liping Yu, Brian D. Fink, and William I. Sivitz

  22. High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria  

Nathanael Miller, Dane Wolf, Nour Alsabeeh, Kiana Mahdaviani, Mayuko Segawa, Marc Liesa, and  Orian Shirihai

 

23. Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism

Dmitri B. Papkovsky and Alexander V. Zhdanov

 

24. Fluorescence-based Assay for Measuring OMA1 Activity

Julia Tobacyk and Lee Ann MacMillan-Crow

 

25. Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay

Wanqing Du, Xiangjun Di, and Qian Peter Su

  26. Extraction of Functional Mitochondria Based on the Membrane Stiffness

Md Habibur Rahman, Qinru Xiao, Shirui Zhao, An-Chi Wei, and Yi-Ping Ho

 

27. A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing

Amanda L. Souza and Gary J. Patti

 

28. A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry

Matej Kohutiar and Adam Eckhardt

 

29. In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans

R. de Boer, R. L. Smith, W. H. De Vos, E. M. M. Manders, andH. van der Spek

 

30. Assessing Impact of Platinum Complexes on Mitochondrial Functions

Suxing Jin and Xiaoyong Wang

 

31. In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models

Roger Springett

 

32. Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a New Molecular Biosensor

Maxime Jacoupy, Emeline Hamon-Keromen, and Olga Corti