Micropropagation in Stevia (Stevia rebaudiana Bertoni)

ISBN-13: 9783659684920 / Angielski / Miękka / 2015 / 112 str.

Rashmi Kumari;Sahay Sanjay;Mishra Praveen Kumar

Opis

The shoot apex, leaf, nodal stem and internodal stem of Stevia rebaudiana, a natural substitute for artificial sweetener having glycosides upto 400 times sweeter than sugar, were cultured on 21 different media having different concentrations and combinations of auxins (2,4-D, IBA, NAA) and cytokinins (KIN, BA) supplemented to MS basal medium. Besides these explants the formed callus and developed shoot were also cultured. The tissue culture experiments resulted in establishment of cultures, swelling of explants, elongation of existing shoot, callus formation, caulogenesis, rhizogenesis and regeneration of plantlets, acclimatization of plantlets and ultimately development of micropropagation protocol.

O autorze

Dr. Rashmi has completed B.Sc(Ag) and M.Sc(Ag) from RAU, Pusa, Bihar in 2005 and 2007.She awarded Doctoral degree in Plant Breeding & Genetics from BAU, Kanke,Ranchi in 2011. Presently she is working as Assistant Professor in Department of Plant Breeding & Genetics,Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India since 2012.