Microinjection: Methods and Applications

ISBN-13: 9781588298843 / Angielski / Twarda / 2008 / 214 str.

David Carroll

Opis

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.

Recenzje

From the reviews:

"Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems ... associated with other master technique (e.g., western blot analysis). ... the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. ... I would like all the readers ... to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)

Spis treści

1. Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation Jill C. Sible and Brian N. Wroble 2. Use of Luciferase Chimaera to Monitor PLC Expression in Mouse Eggs Karl Swann, Karen Campbell, Yuansong Yu, Christopher Saunders, and F. Anthony Lai 3. Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos Jeffrey M. C. Lau and Anthony J. Muslin 4. A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function Katia Cailliau and Edith Browaeys-Poly 5. Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs David J. Carroll and Wei Hua 6. Analysis of Signaling Pathways in Zebrafish Development by Microinjection William H. Kinsey 7. Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complimentary Approaches to Study Protein Function Jane R. Stout, Rania S. Rizk, and Claire E. Walczak 8. DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines Sebastien Chenuet, Madiha Derouazi, David Hacker, and Florian Wurm 9. Bacteriophage fC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels Bryan G. Allen and Daniel L. Weeks 10. Germline Transformation of Caenorhabditis elegans by Injection Pavan Kadandale, Indrani Chatterjee, and Andrew Singson 11. Quantitative Microinjection of Mouse Oocytes and Eggs Douglas Kline 12. Microinjection of Follicle-Enclosed Mouse Oocytes Laurinda A. Jaffe, Rachael P. Norris, Marina Freudzon, William J. Ratzan, and Lisa M. Mehlmann 13. Functional Studies of Regulatory Genes in the Sea Urchin Embryo Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli 14. Exploring the Cytoskeleton during Intracytoplasmic Sperm Injection in Humans Vanesa Y. Rawe and Héctor Chemes 15. Somatic Cell Nuclear Transfer in the Mouse Satoshi Kishigami and Teruhiko Wakayama

Blurb

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.