Microglia: Methods and Protocols

ISBN-13: 9781493996605 / Angielski / Miękka / 2020 / 340 str.

Olga Garaschuk; Alexei Verkhratsky

Spis treści

Part I: Microglia in Health and Disease

 

1. Microglia: The Neural Cells of Non-Neural Origin

            Olga Garaschuk and Alexei Verkhratsky

 

2. Morphology of Microglia across Contexts of Health and Disease

            Julie C. Savage, Micaël Carrier, and Marie-Ève Tremblay

 

3. Physiology of Microglia

            Olga Garaschuk and Alexei Verkhratsky

 

4. Microglia Reactivity: Heterogeneous Pathological Phenotypes

            Hélène Hirbec, François Rassendren, and Etienne Audinat

 

5. Microglia in Neurodegenerative Disorders

            Darío Tejera and Michael T. Heneka

 

6. Physiological Implications of Microglia-Synapse Interactions

            Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, and Junichi Nabekura

 

Part II: In Vitro Analyses of Microglia

 

7. Ultrastructural Analyses of Microglial Interactions with Synapses

            Marie-Ève Tremblay and Ania K. Majewska

 

8. Visualizing Dark Microglia

            Marie-Kim St-Pierre, Maude Bordeleau, and Marie-Ève Tremblay

 

9. Electrophysiological Investigation of Microglia

            Elena Avignone, Giampaolo Milior, Isabelle Arnoux, and Etienne Audinat

 

10. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia

            Annette Masuch and Knut Biber

 

11. Assessment of Parasite-Microglia Interactions In Vitro

            Katherine Figarella, Nestor L. Uzcategui, and Michael Duszenko

 

Part III: Studying Microglia In Vivo

 

12. In Vivo Visualization of Microglia Using Tomato Lectin

            Bianca Brawek, Maria Olmedillas del Moral, and Olga Garaschuk

 

13. Long-Term In Vivo Imaging of Individual Microglial Cells

            Angelos A. Skodras, Jasmin Hefendehl, and Jonas J. Neher

 

14. Analysis of Signaling Mechanisms Regulating Microglial Process Movement

            Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, and Christian Madry

 

15. Measuring Microglial Turnover in the Adult Brain

            Diego Gomez-Nicola, Gemma L. Fryatt, and Katharine E. Askew

 

16. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor

            Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, and Ari Waisman

 

17. Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

            Bianca Brawek and Olga Garaschuk

 

18. Labeling Microglia with Genetically-Encoded Calcium Indicators

            Yajie Liang and Olga Garaschuk

 

19. Calcium Imaging of Microglial Network Activity in Stroke

            Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, and M. Yashar S. Kalani

 

20. In Vivo Two Photon Imaging of Microglial Synapse Contacts

            Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, and Hiroaki Wake

 

21. In Vivo Phagocytosis Analysis of Amyloid Beta

            Darío Tejera and Michael T. Heneka

 

22. Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models

            Nicholas H. Varvel, Richard M. Ransohoff, and Jonas J. Neher

 

Part IV: Omics Analyses of Microglia

 

23. Analysis of the Microglial Sensome

            Suzanne E. Hickman and Joseph El Khoury

 

24. Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue

            Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, and Mélanie Morin-Brureau

Blurb

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.