Microfluidic and Compartmentalized Platforms for Neurobiological Research

ISBN-13: 9781493925094 / Angielski / Twarda / 2015 / 248 str.

Emilia Biffi

Opis

This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and compartmentalized devices for pharmacological research and drug discovery. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Authoritative and practical, Microfluidic and Compartmentalized Platforms for Neurobiological Research provides practical skills needed to fabricate, use microfluidics and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience.

Spis treści

PART I: Designs and Methods

1.      The Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves and their use in Neurobiological Research

 Bryson M. Brewer, Donna J. Webb and Deyu Li

 

2.      A Reliable Reversible Bonding Method for Perfused Microfluidic Devices

Paola Occhetta, Emilia Biffi, and Marco Rasponi

 

3.      Bridging Two Cultures: Minimalistic Networks Prepared by Microfluidic Arraying, and Open Access Compartments for Electrophysiology

Jonathan West, Ngoc-Duy Dinh, Heike Hardelauf, Ya-Yu Chiang, Tracey A. Newman, Mariana Vargas-Caballero, Ayodeji A. Asuni, Katrin Deinhardt, and Martin Arundell

PART II: Axonal Guidance and Manipulation

4.      Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfluidic Chip

Prasanna Sakha, Cecilia Brunello, Joonas Heikkinen, Ville Jokinen, and Henri J. Huttunen

 

5.      Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level

Ting Huang, Tingfei Xi, Wan Qin, Bin Xu, Lina Wei, Lucas Schmidt, Nick Erdman, G. X. Guo, Mauris N Desilva, and Bruce Z. Gao

 

6.      Campenot Cultures and Microfluidics Provide Complementary Platforms for Spatial Study of Dorsal Root Ganglia Neurons

Sara J. Fenstermacher, Maria F. Pazyra-Murphy, and Rosalind A. Segal

PART III: Manipulation Of Synapses

7.      Development of Microfluidic Devices for the Manipulation of Neuronal Synapses

Anika Jain and Martha U. Gillette

 

8.      Use of a 3 Compartment Microfluidic Device to Study Activity Dependent Synaptic Competition

Ainsley Coquinco and Max Cynader

PART IV: Interaction Of Different Cell Populations

9.      Multi-Compartment Neuron-Glia Co-Culture Microsystem

 Jaewon Park, Sunja Kim, Jianrong Li, and Arum Han

10.  Compartmentalized Microfluidic Platforms as Tool of Choice to Study the Interaction Between Neurons and Osteoblasts

Estrela Neto, Diogo Paramos-De-Carvalho, Ana Henriques Lourenço, Paulo Aguiar, and Meriem Lamghari

 

11.  A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit

Katherine A Southam, Anna E King, Catherine A Blizzard, Graeme H Mccormack, and Tracey C Dickson

 

PART V: Pharmacological Manipulation and Screening

12.   Compartmentalized Microfluidics for In Vitro Alzheimer’s Disease Studies

Yufei Ren, Anja Kunze, Philippe Renaud

 

13.  Selective Biochemical Manipulation of Twin Neuronal Networks on Micro Electrode Arrays By Giulia Regalia, Emilia Biffi, Marco Rasponi, and Alessandra Pedrocchi

 

14.  Compartmentalized Synapse Microarray for High-Throughput Screening

Amol D. Jadhav, Wei Li, Zhen Xu, and Peng Shi