Microbial Steroids

ISBN-13: 9781071633847 / Angielski / Twarda / 2023

Opis

This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.

Spis treści

Part I: General Overview

 

1. Current Trends and Perspectives in Microbial Bioconversions of Steroids

            Marina V. Donova

 

Part II: Microbial Screening and Genetic Manipulation

 

2. Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida

            Alejandro Chamizo-Ampudia, Luis Getino, José M. Luengo, and Elias R. Olivera

 

3. Selection of Biodegrading Phytosterol Strains

            María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal

 

4. Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas

            José M. Luengo and Elias R. Olivera

 

5. Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination

            Shikui Song and Zhengding Su

 

Part III: Genetic and Biochemical Analyses

 

6. RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics

            Antonio Rodríguez-García, Alberto Sola-Landa, and Carlos Barreiro

 

7. Bidimensional Analyses of the Intra- and Extra-Cellular Proteomes of Steroid-Producer Mycobacteria

            Carlos Barreiro and Ana M. Ibáñez

 

8. Fungal Sterol Analyses by Gas Chromatography - Mass Spectrometry Using Different Derivatives

            Aurélie Mossion, Isabelle Ourliac-Garnier, and Gaetane Wielgosz-Collin

 

9. Cholesterol Assay Based on Recombinant Cholesterol Oxidase, ABTS, and Horseradish Peroxidase

            Alexey V. Sviridov, Mikhail V. Karpov, Victoria V. Fokina, and Marina V. Donova

 

10. Measuring the Interaction of Sterols and Steroids with Proteins by Microscale Thermophoresis

            Ola El Atab and Roger Schneiter

 

Part IV: Fermentation and Biocatalysis for Steroids Production

 

11. Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products

            Simone Balzer Le, Anna Nordborg, Kjell Domaas Josefsen, Silje Malene Olsen, and Håvard Sletta

 

12. β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors

            Marco P.C. Marques, Jorge Aranda Hernandez, and Pedro Fernandes

 

13. Biocatalysis of Steroids by Mycobacterium sp. in Aqueous and Organic Media

            Carla C.C.R. de Carvalho and Pedro Fernandes

 

14. Scale-Up of Phytosterols Bioconversion into Androstenedione

            Sonia Martínez-Cámara, Manuel de la Torre, José-Luis Barredo, and Marta Rodríguez-Sáiz

 

15. Bioconversion of Phytosterols into Androstenedione by Mycolicibacterium

            Kjell Domaas Josefsen, Anna Nordborg, Simone Balzer Le, Silje Malene Olsen, and Håvard Sletta

 

16. Selective Microbial Conversion of DHEA into 7α-ОН-DHEA

            Vyacheslav Kollerov, Andrei A. Shutov, and Marina V. Donova

 

17. Production of 11α-Hydroxysteroids Derivatives by Corynebacterium glutamicum Expressing the Rhizopus oryzae Hydroxylating System

            Beatriz Galán, Carmen Felpeto-Santero, and José Luis García

 

18. Obtaining of 24-Norchol-4-Ene-3,22-Dione from Phytosterol with Mutants of Mycolicibacterium neoaurum

            Dmitry V. Dovbnya, Tanya V. Ivashina, Sergey M. Khomutov, Andrei A. Shutov, Natalia O. Deshcherevskaya, and Marina V. Donova

 

19. Stabilization of Enzymes by Using Thermophiles

            Ana-Luisa Ribeiro, Mercedes Sánchez, Sandra Bosch, José Berenguer, and Aurelio Hidalgo

 

20. Stigmasterol Removal by an Aerobic Treatment System

            Soledad Chamorro, Claudia Xavier, and Gladys Vidal

 

Part V: Steroids Application

 

21. Glucocorticoid Effect in Cancer Patients

            María Marta Blanco-Nistal and Jesús Antonio Fernández-Fernández