Microbial Plant Pathogens-Detection and Disease Diagnosis:: Fungal Pathogens, Vol.1

ISBN-13: 9789400789760 / Angielski / Miękka / 2014 / 291 str.

P. Narayanasamy

Opis

Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.

Recenzje

From the reviews:

"The first volume of the Microbial plant pathogens - Detection and disease diagnosis focuses on fungal pathogens. ... All chapters are summarized in the abstract, well referenced and focused on providing concerns of techniques and improvements of research. Therefore, if you are interested in the methods for detection and diagnosis, this is certainly a book you must have. The book should be available to every mycologist and students studying any aspect of plant pathology. Every library in the world should also have this book." (Dhanushka Udayanga and Dimuthu S. Manamgoda, Fungal Diversity, April, 2012)

Spis treści

Volume 1 Chapter 1 Introduction 1.1 Microbial plant pathogens as a major limiting factor of crop production 1.2 Discovery of fungi as plant pathogens 1.3 Detection of fungal plant pathogens and disease diagnosis References Chapter 2 Detection of Fungal Pathogens in Plants 2.1 Detection of fungal pathogens in plant organs 2.1.1 Biological methods 2.1.2 Pathogenicity and host range 2.1.3 Biochemical methods 2.1.4 Immunoassays 2.1.5 Nucleic acid-based detection techniques 2.2 Detection of fungal pathogens in seeds and planting materials 2.2.1 Detection of fungal pathogens in seeds 2.2.2 Detection of fungal pathogens in propagative planting materials 2.2.3 Detection of fungal pathogens in postharvest produce Appendix References Chapter 3 Detection of Fungal Pathogens in the Environment 3.1 Detection of fungal pathogens in soil 3.1.1 Bioassays 3.1.2 Immunoassays 3.1.3 Nucleic acid-based techniques 3.2 Detection of fungal pathogens in water 3.2.1 Immunoassays 3.2.2 Nucleic acid-based techniques 3.3 Detection of fungal pathogens in air 3.4 Detection of fungal pathogens in alternative host plants Appendix References Chapter 4 Assessment of Variability in Fungal Plant Pathogens 4.1 Methods of assessment of variability in fungal pathogens 4.1.1 Assessment of variations in biological characteristics 4.1.2 Assessment of variations in biochemical characteristics 4.1.3 Assessment of variations in immunological characteristics 4.1.4 Assessment of variations in genomic characteristics 4.2 Assessment of variability in sensitivity to chemicals 4.2.1 Assessment of variations in biological characteristics 4.2.2 Assessment of variations in genetic characteristics Appendix References Chapter 5 Diagnosis of Fungal Diseases of Plants 5.1 Choice of diagnostic tests for fungal pathogens 5.1.1 Conventional methods 5.1.2 Molecular methods 5.2 Agencies involved in disease diagnosis 5.2.1 Disease diagnostic centers 5.2.2 Plant quarantines References

Blurb

Plants are infected by different microbial pathogens, of which fungal pathogens form the highly evolved and earliest recognized group. The morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Pathogenicity on a set of differential host plant species or crop cultivars has been useful for the identification of physiologic races existing within a morphologic species/variety. As the techniques based on the above mentioned characteristics require long time and yield inconsistent results, the need for the development of techniques with greater accuracy, reliability, reproducibility and capable of providing the results rapidly was realized. Immunoassays have been shown to be effective in detecting several fungal pathogens present in plants, planting materials, soil, water and air. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization of probes with complementary sequences of the target pathogen(s) or amplification of specific sequences of the target pathogen DNA/fragment have been demonstrated to be the most efficient among the diagnostic techniques currently available. This volume provides exhaustive information on various methods of detection of fungal pathogens and the diagnosis of the diseases caused by them based on extensive literature search that will be useful to the researchers, teachers and graduate students of different disciplines of biological sciences, in addition to the extension pathologists, personnel of plant quarantines and certification programs. Presentation of a large number of protocols is a unique feature of this volume to facilitate selection of appropriate techniques for different investigations.