Microbial Plant Pathogens-Detection and Disease Diagnosis

ISBN-13: 9789048197538 / Angielski / Twarda / 2010 / 321 str.

P. Narayanasamy

Opis

With nucleic acid-based techniques being the most efficient tool for detecting plant viruses, this text includes numerous protocols and distils the extensive literature in the field, offering a comprehensive resource for researchers in the biological sciences.

Spis treści

Volume 3 Chapter 1 Introduction 1.1 Microbial plant pathogens as a major limiting factor of crop production 1.2 Discovery of viruses and viroids as plant pathogens 1.3 Detection of viral and viroid plant pathogens and disease diagnosis References Chapter 2 Detection of Viral and Viroid Pathogens in Plants 2.1 Detection of viruses in plant organs 2.1.1 Plant virus taxonomy 2.1.2 Biological methods 2.1.3 Morphological characteristics 2.1.4 Physical and biochemical characteristics 2.1.5 Immunoassays 2.1.6 Nucleic acid-based techniques 2.2 Detection of viroids in plant organs 2.2.1 Molecular characteristics 2.2.2 Biological methods 2.2.3 Physico-chemical methods 2.2.4 Immunoassays 2.2.5 Nucleic acid-based techniques 2.3 Detection of viral pathogens in seeds and planting materials 2.3.1 Detection of viruses in seeds 2.3.2 Detection of viruses in planting materials 2.4 Detection of viroids in seeds and planting materials 2.4.1 Detection of viroids in seeds 2.4.2 Detection of viroids in planting materials Appendix References Chapter 3 Detection of Viral and Viroid Plant Pathogens in the Environment 3.1 Detection of plant viruses in environment 3.1.1 Detection of plant viruses in soil 3.1.2 Detection of plant viruses in water 3.1.3 Detection of plant viruses in air 3.1.4 Detection of plant viruses in alternative host plant species 3.2 Detection of viroids in the environment Appendix References Chapter 4 Assessment of Variability in Plant Viral and Viroid Pathogens 4.1 Assessment of variability in plant viral pathogens 4.1.1 Assessment of variations in biological characteristics 4.1.2 Assessment of variations in physico-chemical characteristics 4.1. 3 Assessment of variations in immunological characteristics 4.1.4 Assessment of variations in genomic characteristics 4.2 Assessment of variability in viroid pathogens 4.2.1 Assessment of variations in nucleic acid characteristics Chapter 5 Diagnosis of Viral and Viroid Diseases 5.1 Choice of diagnostic tests for viral diseases 5.1.1 Biological tests 5.1.2 Physico-chemical tests 5.1.3 Immunoassays 5.1.4 Nucleic acid-based techniques 5.2 Choice of diagnostic tests for viroid diseases 5.2.1 Biological tests 5.2.2 Nucleic acid-based techniques 5.3 Agencies involved in disease diagnosis 5.3.1 Disease diagnostic centers 5.3.2 Plant quarantines References

Blurb

Viruses, with the primitive structural features are capable of causing diseases in all life forms on the earth. With limited morphological characteristics of the virus particles and lack of physiological functions, the need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays based on the antigenic properties of viral capsid proteins have been successfully applied for the detection of viruses in the symptomatic and asymptomatic crop and weed host plant species as well as in the vectors that are involved in the transmission of the viruses infecting crop plants. Development of monoclonal antibody technology has enhanced the sensitivity and specificity of immunoassays especially applied for the differentiation of strains of viruses with different virulence levels. Nucleic acid-based techniques have been demonstrated to have higher levels of sensitivity, specificity, reliability, reproducibility and rapidity. The viroids with only an RNA molecule have been detected by techniques based on the biological characteristics in certain cases. Application of immunoassays for viroid detection is not feasible because of the absence of a protein component that is present in the viruses. Nucleic acid-based techniques are the most reliable and sensitive tests for detection, identification and differentiation of viroids present in plants and planting materials. With the aim of providing a comprehensive knowledge of detection of viruses and viroids and diagnosis of the diseases caused by them, information distilled from extensive literature search is presented to fulfil the requirements of the researchers, teachers and graduate students of various disciplines of biological sciences. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume to enable the scientists to select the suitable methods for the various investigations.