Microbial-Based Biopesticides: Methods and Protocols

ISBN-13: 9781493981786 / Angielski / Miękka / 2018 / 224 str.

Travis R. Glare; Maria E. Moran-Diez

Spis treści

1 What are Microbial-based Biopesticides?

María E. Morán-Diez and Travis R. Glare

 

Part I: Screening, Isolation and Identification of Potential Biological Control Agents

2. Isolation and Mass Production of Trichoderma

Artemio Mendoza-Mendoza, Annabel Clouston, Jin-Hua Li, Maria Fernanda Nieto-Jacobo, Nicholas Cummings, Johanna Steyaert,  and Robert Hill

 

Part II: Evaluating Mode of Action of Microorganisms

3. Methods for the Evaluation of the Bioactivity and Biocontrol Potential of Species of Trichoderma

Johanna Steyaert, Emily Hicks, Janaki Kandula, Diwakar Kandula, Hossein Alizadeh, Mark Braithwaite, Jessica Yardley, and Artemio Mendoza-Mendoza

 

Part III:  Mass Production and Formulations: Bacteria

4. Purification of the Yersinia Entomophaga Yen-TC Toxin Complex using Size Exclusion Chromatography

Sandra A. Jones and Mark R. H. Hurst

 

5. Coated Solid Substrate Microbe Formulations: Pseudomonas spp. and zeolite

Craig R. Bunt, Sally Price, John Hampton, and Scott Stelting

 

Part IV: Mass Production and Formulations: Fungi

6. Production of Conidia by the Fungus Metarhizium Anisopliae using Solid-state Fermentation

Octavio Loera-Corral, Javier Porcayo-Loza, Roberto Montesinos-Matias, and Ernesto Favela-Torres

 

7. Liquid Culture Production of Fungal Microsclerotia

Mark A. Jackson and Angela R. Payne

 

8. Isolation and Assessment of Stability of Six Formulations of Entomopathogenic Beauveria Bassiana

Lizzy A. Mwamburi

 

Part V: Mass Production and Formulations: Viruses

9. Cell Culture for Production of Insecticidal Viruses

Steven Reid, Leslie C. L. Chan, Leila Matindoost, Charlotte Pushparajan, and Gabriel Visnovsky

 

<Part VI: Mass Production and Formulations: Nematodes

10. Formulation of Nematodes

Arne Peters

 

11. In vivo Production of Entomopathogenic Nematodes

David I. Shapiro-Ilan, Juan A. Morales-Ramos, and M. Guadalupe Rojas

 

Part VII: Monitoring of Applied Microbes

12. Detection and Quantification of the Entomopathogenic Fungal Endophyte Beauveria Bassiana in Plants by Nested and Quantitative PCR

Inmaculada Garrido-Jurado, Blanca B. Landa, and Enrique Quesada-Moraga

 

13 Plant Tissue Preparation for the Detection of an Endophytic Fungus in Planta

Aimee C. McKinnon

 

VIII: Quality Control, Safety, and Registration

14. Measuring Chitinase and Protease Activity in Fungal Cultures

Peter Cheong

 

15. Analytical Methods for Secondary Metabolite Detection

Judith Taibon and Hermann Strasser

 

16. Development of Biopesticides and Future Opportunities

Travis R. Glare, Roma L. Gwynn, and Maria E. Moran-Diez

 

Blurb

This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy.  Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Microbial-Based Biopesticides aims to ensure successful results in the further study of this vital field.