Microarray Methods for Drug Discovery

ISBN-13: 9781607616627 / Angielski / Twarda / 2010 / 285 str.

Sridar V. Chittur

Opis

The postgenomic era presents a multitude of challenges for scientists in all areas of science. The information overload from new discoveries in genomics and proteomics highlight how little we really know about the functioning of a cell. The advent of Next-Generation Sequencing technologies promises to make our genetic blueprint available to the common man. The availability of the plethora of biological information has lead to the devel- ment of new areas of science and the coining of new "omics" terms including transcr- tomics, methylomics, toxicogenomics, pharmacogenomics, metabolomics, lipidomics, and so on. Remarkable research is being conducted to understand the various aspects of human health and how processes like histone modifcations, promoter usage, alternative splicing, posttranscriptional, and posttranslational modifcations contribute to disease. The advent of systems biology has unifed chemists and biochemists alike in the struggle to eradicate or treat human disease. Microarrays have blossomed into a fast developing and cutting-edge technology that promises to become a major component of personalized medicine. The 1990s witnessed a boom in many areas including genome sequencing, combinatorial chemistry, and c- puters, all of which have contributed to the development of microarray technology from its infancy into a mature tool. The growing potential of this tool is evident from the n- ber of publications since 1991 when Fodor et al. of Affymax (now Affymetrix) frst described the microarray prototype.

Recenzje

From the reviews:

"Microarray Methods for Drug Discovery is volume number 632 of the Springer Protocols Methods in Biology Series. ... it provides detailed experimental protocols for the use of microarrays in a variety of applications. ... chapters are obviously highly bespoke and would be of real value to laboratories wishing to establish the method. Finally, the microarray area is now so broad that the non-nucleic acid application of microarrays would justify a methods book in its own right." (James Sidaway, BTS Newsletter, Issue 37, Winter, 2010)

"Methods in Microarrays provide scientists with a complete resource on the most effective tools available for maximizing microarray data in biochemical methods. ... Microarrays are extensively used in the effects of compounds on gene expression in cells. This book aims at enlightening individuals of all levels of experience about some of the most common applications of microarrays in drug discovery and development. This book will serve as a reference for students and scientists ... ." (Veni Maria Andres Felli, Brazilian Journal of Pharmaceutical Sciences, May, 2010)

Spis treści

1. Multicenter Clinical Sample Collection for Microarray Analysis Tony S. Mondala, Daniel R. Salomon, and Steven R. Head 2. Selective Isolation of Total RNA from Mouse Melanoma Subsets Using Fluorescence Activated Cell Sorting Scott Tighe and Matthew A. Held 3. Microarray Analysis of Embryonic Stem Cells and Differentiated Embryoid Bodies Alexander C. Zambon and Christopher S. Barker 4. Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST Arrays Sita Subbaram, Marcy Kuentzel, David Frank, C. Michael DiPersio, and Sridar V. Chittur 5. Profiling microRNA Expression with the Illumina BeadChip Platform Julissa Tsao, Patrick Yau, and Neil Winegarden 6. TaqMan® Array Cards in Pharmaceutical Research David N. Keys, Janice K. Au-Young, and Richard A. Fekete 7. DMET™ Microarray Technology for Pharmacogenomics-Based Personalized Medicine James K. Burmester, Marina Sedova, Michael H. Shapero, and Elaine Mansfield 8. The Use of Microarray Technology for Cytogenetics Bassem A. Bejjani, Lisa G. Shaffer, and Blake C. Ballif 9. PCR/LDR/Universal Array Platforms for the Diagnosis of Infectious Disease Maneesh Pingle, Mark Rundell, Sanchita Das, Linnie M. Golightly, and Francis Barany 10. RIP-CHIP in Drug Development Ritu Jain, Francis Doyle, Ajish D. George, Marcy Kuentzel, David Frank, Sridar V. Chittur, and Scott A. Tenenbaum 11. ChIPing Away At Global Transcriptional Regulation Kelly Jackson, James Paris, and Mark Takahashi 12. HELP (HpaII Tiny Fragment Enrichment by Ligation-Mediated PCR) Assay for DNA Methylation Profiling of Primary Normal and Malignant B Lymphocytes Rita Shaknovich, Maria E. Figueroa, and Ari Melnick 13. High-Throughput Screening of Metalloproteases Using SmallMolecule Microarrays Mahesh Uttamchandani 14. Metabolic Enzyme Microarray Coupled with Miniaturized Cell-Culture Array Technology for High-Throughput Toxicity Screening Moo-Yeal Lee, Jonathan S. Dordick, and Douglas S. Clark 15. Use of Tissue Microarray to Facilitate Oncology Research Panagiotis Gouveris, Paul M. Weinberger, and Amanda Psyrri 16. Small Molecule Selectivity and Specificity Profiling Using Functional Protein Microarrays Peter R. Kraus, Lihao Meng, and Lisa Freeman-Cook 17. Production and Application of Glycan Microarrays Julia Busch, Ryan McBride, and Steven R. Head

Blurb

While the utilization of microarrays for gene expression studies has been widely published, Microarray Methods for Drug Discovery describes the use of this technology for a multitude of other vital applications. Covering the field of drug discovery through its various aspects, including high throughput screening, target identification, drug metabolism and toxicity screening as well as clinical sample handling, this volume also addresses new emerging fields like miRNA profiling, ribonomic and glycomic profiling. All chapters, contributed by experts in the field, follow the highly successful Methods in Molecular Biology™ series format, which is comprised of brief introductions, lists of required materials and reagents, readily reproducible, step-by-step lab protocols, along with detailed tips on troubleshooting and avoiding known pitfalls, making this book an easy read for investigators at all levels of expertise.

Authoritative and cutting-edge, Microarray Methods for Drug Discovery provides an ideal desk reference for scientists who wish to use microarray technology in order to better understand a variety of biological interactions in their respective fields.