Microarray Innovations: Technology and Experimentation

ISBN-13: 9781420094480 / Angielski / Twarda / 2009 / 314 str.

Gary Hardiman; Gary Hardiman

Opis

In recent years, high-density DNA microarrays have revolutionized biomedical research and drug discovery efforts by the pharmaceutical industry. Their efficacy in identifying and prioritizing drug targets based on their ability to confirm a large number of gene expression measurements in parallel has become a key element in drug discovery. Microarray Innovations: Technology and Experimentation examines the incredibly powerful nature of array technology and the ways in which it can be applied to understanding the genomic basis of disease. Explores a myriad of applications in use today This volume explores recent innovations in the microarray field and tracks the evolution of the major platforms currently used. The international panel of contributors presents a survey of the past five years research and advancements in microarray methods and applications and their usage in drug discovery and biomedical research. The contributions discuss improvements in automation (array fabrication and hybridization), new substrates for printing arrays, platform comparisons and contrasts, experimental design, and data normalization and mining schemes. They also review epigenomic array studies, electronic microarrays, comparative genomic hybridization, microRNA arrays, and mutational analyzes. In addition, the book provides coverage of important clinical diagnostic arrays, protein arrays, and neuroscience applications. Examines improved methodologies As microarrays have evolved steadily over time from archetypical in-house complementary DNA (cDNA) arrays to robust commercial oligonucleotide platforms, there has been a migration to higher density biochips with increasing content and better analytical methodologies. This compendium summarizes the vast advances that have been made in this technology, highlighting the supreme advantages of microarray-based approaches in the field of biomedical research. Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry."

Spis treści

Introduction to Gene Expression Profiling with DNA Microarray Technology. Introduction to Large-Scale Gene Expression Data Analysis. The Statistical Design and Analysis of Gene Expression Data. Laboratory Automation for Microarray Experimentation. How to Evaluate a Microarray Scanner. The Role of Substrates in Microarray Experimentation and How to Choose the Correct Coating for Microarraying. Towards Highly Efficient Automated Hybridizations. Characterization of Microarray Hybridization Stoichiometry. Data Normalization Selection. Methods for Assessing Microarray Performance. Comparison of Different Normalization Methods for Applied Biosystems Expression Array System. A Systematic Comparison of Gene Expression Measurements Across Different Hybridization-Based Technologies. DNA Methylation Arrays: Methods and Analysis. Electronic Microarrays: Progress toward DNA Diagnostics. New Applications for Microarrays. Development of an Oligonucleotide Microarray for Mutational Analysis Using Single or Multiple Sample Hybridization. Development of an Integrated Molecular Diagnostic Test to Identify Respiratory Viruses. Microarrays in Neuroscience. Optimization of Protein Array Fabrication for Establishing High Throughput Ultra-Sensitive Microarray Assays for Cancer Research. Index.

 

O autorze

Gary Hardiman