Microarray Analysis of the Physical Genome: Methods and Protocols

ISBN-13: 9781603271912 / Angielski / Twarda / 2009 / 224 str.

Jonathan R. Pollack

Opis

In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome. In Microarray Analysis of the Physical Genome: Methods and Protocols, experts in the field cover DNA microarray applications for the detection and characterization of genomic DNA-associated copy number alteration, loss of heterozygosity (LOH), cytosine methylation, protein binding sites, regulatory elements, and replication timing, with an emphasis on higher eukaryote (animal) and cancer genomes. Written in the highly successful Methods in Molecular BiologyT series format, chapters include a brief introduction to their respective subjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Microarray Analysis of the Physical Genome: Methods and Protocols is of great value to the molecular biologist or computational biologist interested in understanding the principles of these analyses or in planning future experiments using microarrays to characterize the physical genome.

Spis treści

Chapter 1. Introduction Jonathan R. Pollack Chapter 2. Comparative Genomic Hybridization on BAC Arrays Bradley P. Coe, William W. Lockwood, Raj Chari, and Wan L. Lam Chapter 3. Comparative Genomic Hybridization on Spotted Oligonucleotide Microarrays Young H. Kim and Jonathan R. Pollack Chapter 4. Comparative Genomic Hybridization by Representational Oligonucleotide Microarray Analysis Robert Lucito, and James Byrnes Chapter 5. Application of Oligonucleotides Arrays for Coincident Comparative Genomic Hybridization, Ploidy Status and Loss of Heterozygosity Studies in Human Cancers John K. Cowell, and Ken C. Lo Chapter 6. Molecular Inversion Probe Assay for Allelic Quantitation Katrina Welch, and Hanlee Ji Chapter 7. A Whole Genome Amplification Protocol for a Wide Variety of DNAs, Including those from Formalin-Fixed and Paraffin-Embedded Tissue Pamela L. Paris Chapter 8. Algorithms for Calling Gains and Losses in Array CGH Data Pei Wang Chapter 9. Methylation Analysis by Microarray Daniel E. Deatherage, Dustin Potter, Pearlly S. Yan, Tim H.-M. Huang, and Shili Lin Chapter 10. Methylation Analysis by DNA Immunoprecipitation (MeDIP) Emily A. Vucic, Ian M. Wilson, Jennifer M. Campbell, and Wan L. Lam Chapter 11. Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functional Genomic Studies Jérôme Eeckhoute, Mathieu Lupien, and Myles Brown Chapter 12. ChIP-Chip: Algorithms for Calling Binding Sites Clifford A. Meyer and X. Shirley Liu Chapter 13. Mapping Regulatory Elements by DNaseI Hypersensitivity Chip (DNase-Chip) Yoichiro Shibata, and Gregory E. Crawford Chapter 14. Microarray Analysis of DNA Replication Timing Neerja Karnani,Christopher M. Taylor, and Anindya Dutta Chapter 15. Integration of Diverse Microarray Data Types Keyan Salari and Jonathan R. Pollack

Blurb

In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome. In Microarray Analysis of the Physical Genome: Methods and Protocols, experts in the field cover DNA microarray applications for the detection and characterization of genomic DNA-associated copy number alteration, loss of heterozygosity (LOH), cytosine methylation, protein binding sites, regulatory elements, and replication timing, with an emphasis on higher eukaryote (animal) and cancer genomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include a brief introduction to their respective subjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Microarray Analysis of the Physical Genome: Methods and Protocols is of great value to the molecular biologist or computational biologist interested in understanding the principles of these analyses or in planning future experiments using microarrays to characterize the physical genome.