MicroRNA Profiling: Methods and Protocols

ISBN-13: 9781071628225 / Angielski / Twarda / 2022 / 252 str.

Sweta Rani

Opis

This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Spis treści

1.      miRNA Biogenesis and Regulation of Diseases: An updated Overview

Anchal Vishnoi and Sweta Rani

2.      Exosomal MicroRNA profiling

Neda Rahimian, Javid Sadri Nahand, Michael R Hamblin4, Hamed Mirzaei

3.      MicroRNA expression profiling using Agilent One-Color Microarray

Carmela Dell’Aversana, Giulia Sgueglia,Nunzio Del Gaudio,Lucia Altucci

4.      A simple method for profiling and analysing microRNAs from small volume samples using a qPCR-based platform

Aisling Leavy and Eva M Jimenez-Mateos

5.      Exosomal microRNAs: comprehensive methods from exosome isolation to miRNA extraction and purity analysis

D’Agostino Erika, Muro Annamaria, Sgueglia Giulia, Massaro Crescenzo, Dell’Aversana Carmela and Altucci Lucia

6.      Detection of MicroRNAs in Brain Slices Using In Situ Hybridization

Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, and Eva M. Jimenez-Mateos

7.      MicroRNA profiling of cell lines and xenografts by quantitative PCR

Ariadna Boloix, Miguel F. Segura

8.      Assessment of basic biological functions exerted by miRNAs

Ellen King, John Nolan, Olga Piskareva

9.      Serum microRNAs Profiling in Age-Related Macular Degeneration

Hanan ElShelmani, David Keegan, Sweta Rani

10.    Exosomal microRNA discovery in Age-Related Macular Degeneration

Hanan Elshelmani, Sweta Rani

11.    MicroRNA profiling using a PCR based method

Giuliana de A. Ferronato, Marcela B. Cerezetti, Alessandra Bridi, Cibele M. Prado, Gislaine dos Santos, Natália M. Bastos, Paola M. da S. Rosa, Juliana G. Ferst, and Juliano C. da Silveira

12.    Guidelines on designing microRNA sponges: from construction to stable cell line

Manoela Marques Ortega, Hakim Bouamar

13.    Evaluating single-nucleotide variants in microRNAs targeting sites and mature microRNAs in vitro cell culture by luciferase reporter gene assays

Jéssica Silva dos Santos1 Gabriel Alves Bonafé1 Gustavo Jacob Lourenço, Manoela Marques Ortega

14.    Assessment of cell cytotoxicity in 3D biomaterial scaffolds following miRNA transfection

Elizabeth Sainsbury, Lara Costard, Fergal J. O’Brien, Caroline M. Curtin

15.    Evaluation of miRNA expression in 3D in vitro scaffold-based cancer models

Catherine Murphy, Ciara Gallagher, and Olga Piskareva

16.    Bioinformatics Analysis of MiRNA sequencing Data

Hrishikesh A. Lokhand

17.    Plant microRNA identification and annotation using deep sequencing data

Zheng Kuang, Yongxin Zhao, and Xiaozeng Yang