Methods in Tau Cell Biology: Volume 141

ISBN-13: 9780128124987 / Angielski / Twarda / 2017 / 342 str.

Stuart Feinstein; Nichole Lapointe

Opis

Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.

• Covers sections on Tau Cell Biology
• Written by experts in the field of cell biology
• Includes cutting-edge materials

Spis treści

1. Recombinant tau expression and purification Stuart Feinstein 2. In vitro MT dynamics and MT ends Nichole LaPointe 3. Methods related to investigating tau structure and MT bundling Cyrus R. Safinya 4. Neurite outgrowth and retraction Brett Cook 5. Methods related to studying tau fragmentation Adrianna Ferrerira 6. Isoform-specific differences in tau in vitro aggregation Truman C. Gamblin 7. Cosedimentation assays of microtubule binding Holly Goodson 8. Methods to study tau aggregation Songi Han 9. Methods to study tau phosphorylation Diane Hanger 10. Structure of tau oligomers Nicholas M. Kanaan 11. Use of mouse models for studies of pathological tau action Virginia Lee and Sneha Narasimhan 12. Application of FRET and FCS to study tau structure-function Elizabeth Rhoades 13. Use of the fly system to study tau action Michelle Steinhilb 14. Single molecule assays with motor proteins Christopher Berger 15. Tau clearance mechanisms in primary neurons with a focus on autophagy Gail Johnson 16. Structural studies of tau Eckhard Mandelkow 17. TIRF assays of tau effects on MT and actin cytoskeletons in vitro Isabelle Arnal 18. TIRF assays of tau interactions with EB1 Isabelle Arnal 19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated viruses Susanne Wegmann