Methods in Practical Laboratory Bacteriology

ISBN-13: 9780849386923 / Angielski / Miękka / 1994 / 176 str.

Henrik Chart

Opis

Methods in Practical Laboratory Bacteriology provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE).

Spis treści

Bacterial Fractionation and Membrane Protein Characterization (H. Chart). Lipopolysaccharide: Isolation and Characterization (H. Chart). Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) for the Separation and Resolution of Bacterial Components (H. Chart). Reaction of Antibodies with Bacterial Components Using Immunoblotting (H. Chart).The Use of Enzyme-Linked Immunosorbent Assay (ELISA) in Bacteriology (H. Chart). Environmental Regulation of Bacterial Characteristics (P. Stevenson and E. Griffiths). Testing for Resistance to Antimicrobial Drugs (J.A. Frost). Pulsed-Field Gel Electrophoresis of Bacterial DNA (M.E. Kaufmann and T.L. Pitt). Extraction and Fingerprinting of Bacterial Plasmids (N. Woodford, A.P. Johnson, and E.J. Threlfall). Non-Radioactive Digoxigenin-Labeled DNA Probes (A. Thomas). Ribotyping of Bacterial Genomes (M.E. Kaufmann, D.G. Pitcher, and T.L. Pitt). Appendix. References.Sources of Equipment and Chemicals.
Glossary. Index

O autorze

Henrik Chart