Methods in Mouse Atherosclerosis

ISBN-13: 9781493949359 / Angielski / Miękka / 2016 / 400 str.

Vicente Andres; Beatriz Dorado

Spis treści

1. Use of Mouse Models in Atherosclerosis Research

Godfrey S. Getz and Catherine A. Reardon

 

2. Cytokines and Immune Responses in Murine Atherosclerosis

P.J.H. Kusters and E. Lutgens

 

3. Intravital Microscopy for Atherosclerosis Research

Remco T. A. Megens and Oliver Soehnlein

 

4. Non-invasive Molecular Imaging of Mouse Atherosclerosis

Martina A. McAteer and Robin P. Choudhury

 

5. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado

 

6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

Inés Pineda-Torra, Matthew Gage, Alba de Juan, and Oscar M. Pello

 

7. Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies

Pedro Molina-Sánchez and Vicente Andrés

 

8. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay

Ricardo Villa-Bellosta and Magda R. Hamczyk

 

9. Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta

Prashanthi Menon and Edward A. Fisher

 10. Immunostaining of Lymphocytes In Mouse Atherosclerotic PlaqueAnton Gisterå and Daniel F.J. Ketelhuth

 

11. Flow Cytometric Analysis of Immune Cells Within Murine Aorta

Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina

 

12. In Vitro Differentiation of Naïve CD4⁺ T cells: A Tool for Understanding the Development of Atherosclerosis

Salvador Iborra and Jose M. Gonzalez-Granado

 

13. Quantification of Apoptosis in Mouse Atherosclerotic Lesions

Nichola L Figg and Martin R Bennett

 

14. Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions

José J. Fuster

15. Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport

Joan Carles Escolà-Gil, Miriam Lee-Rueckert, David Santos, Lídia Cedó, Francisco Blanco-Vaca , and Josep Julve

 

16. In vitro Macrophage Phagocytosis Assay

Magda R. Hamczyk, Ricardo Villa-Bellosta, Vicente Andrés

 

17. Glucose and Insulin Tolerance Tests in the Mouse

Ángela Vinué and Herminia González-Navarro

 

18. Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries

Lara del Campo and Mercedes Ferrer

 

19. Pressure Myography to Study the Function and Structure of Isolated Small Arteries

Olav L. Schjørring, Rune Carlsson,  and Ulf Simonsen

 

20. Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays

Changjun Yin, Sarajo Mohanta , Zhe Ma, Christian Weber, Desheng Hu, Falk Weih, and Andreas Habenicht

 

21. Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR

José Rivera-Torres

 

22. Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis

Vinatha Sreeramkumar and Andrés Hidalgo

 

23. Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse

Chen Y.C.^, Rivera J., and Peter K.

 

24. Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions

Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman

 

25. Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis

Raphaël Chèvre

 

26. Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies

Cristina Rius and María J. Sanz

 

27. Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis

Alicia Arranz, Markus Rudin, Carlos Zaragoza, and Jorge Ripoll

 

28. In vivo ¹⁸F-FDG-PET Imaging in Mouse Atherosclerosis

Jesús Mateo, Izaskun Bilbao, Juan José Vaquero, Jesús Ruiz-Cabello, and Samuel España

 

29. Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta

Jesús Mateo, Marina Benito, Samuel España, Javier Sanz, Jesús Jiménez-Borreguero Valentín Fuster, and Jesús Ruiz-Cabello

Blurb

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors’ personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.