Methods in Gene Technology, Volume 2

ISBN-13: 9781559382649 / Angielski

Dale / McAslan / Dussek

Opis

Gene probes, whether RNA or DNA, have played a central role in the rapid development of molecular biology. The wide variety of applications is matched by a considerable diversity in the methods used for generating probes, a complete account of which would be very difficult to make. Instead, this second volume in the series combines a selection of newer gene probe procedures with a review of the most important established methods, together with some examples of the ways in which gene probes can be applied. In doing so, the book aims to act not only as an introductory manual for newcomers to the field, but also as a means of broadening the horizons of existing researchers.

Spis treści

Contents. List of Contributors. Introduction. Purification of DNA (J.W. Dale). Electrophoretic Separation of Nucleic Acids (P.G. Sanders0. Preparative Gel Electrophoresis (J.C. Maule). Plasmid Isolation Procedures (A.I. Knight). Plasmid Isolation: column Methods (T.J. Hellyer0. Isolation of Large Plasmids (G.L. Jones and P.A. Williams). Isolation of Linear and Single-Strand Plasmid DNA (J.C. Alonso). Preparation of Bacteriophage &lgr; DNA (E. Norman). Purification of Viral RNA (J.E. Whitby, A.D.T. Barrett, A.D. Jennings, and P. Johnstone). Large Scale DNA Sequencing by Manual Methods (A.J. Davison and E.A.R. Telford). Pulsed-Field Gel Electrophoresis: Applications to Bacteria (P.R. Stewart, W. El-Adhami, and B. Inglis). Pulsed-Field Gel Electrophoresis: Large Scale Restriction Maps (N. Okahashi and T. Koga). Pulsed-Field Gel Electrophoresis: Isolation and Analysis of Large Linear Plasmids (H. Kinashi). Detection of Mutations by PCR-Single-Strand Conformation Polymorphism (A. Telenti). Analysis of DNA Supercoiling (K. Drlica, M. Malik, J.Y. Wang, A. Sasiak and R.R. Sinden). Gel Retardation Assays and DNA Footprinting (I.S. Goping, D.A. Underhill, and G.C. Shore). Southwestern Blot (G. Corte and M.T. Corsetti). Genomic Fingerprinting Using the PCR-Random Amplified Polymorphic DNA Technique (S.V.P.S. Silva and J.D. Procunier). PCR Walking (M.G. Burdon and J.J. Willoughby). Index.