Methods in Bone Biology

ISBN-13: 9781475771220 / Angielski / Miękka / 2013 / 314 str.

T. Arnett; Brian Henderson

Opis

Methods in Bone Biology is unique in being devoted to describing the methodology used by bone researchers. This book describes in detail the techniques of cell and organ culture used in the study of bone and bone cell function and the techniques used to monitor the skeleton and skeletal remodelling both in clinical and experimental settings.

Spis treści

Culture of cells of the osteoblast lineage. In vitro models for osteoclast recruitment. Isolation and culture of osteoclasts and osteoclast resorption assays. Bone organ cultures. Methods for studying cell death in bone. Models for mechanical loading of bone and bone cells in vivo and in vitro. Bone histomorphometry. The application of immunocytochemistry and in situ hybridization to cryostat sections of undecalcified bone. Biochemical markers of bone turnover. Radiographic measurement of bone turnover: microfocal radiography. Bone mineral measurements by DXA in animals. Animal models for the investigation of actions of factors on bone metabolism.