Methods for the Discovery and Characterization of G Protein-Coupled Receptors

ISBN-13: 9781493958511 / Angielski / Miękka / 2016 / 428 str.

Craig W. Stevens

Opis

This book presents the latest methods and technology used to characterize and discover novel mechanisms of G protein-coupled receptors (GPCRs) which, in many cases, can be used directly to design experiments for the reader's particular GPCR of interest.

Recenzje

From the reviews:

"Methods for the Discovery and Characterization of G Protein-Coupled Receptors is an excellent reference text for anyone concerned with the dynamic life of GPCRs in living tissues. The book is suitably organized to cater to a wide audience, and the material is presented in a logical framework to permit understanding of major concepts by a novice. ... This would be a particularly valuable book for any structural biologist looking to build complexity into their experimental system to account for in vivo phenomena." (Michelle O'Malley, ChemBioChem, Vol. 13 (6), April, 2012)

Spis treści

Part I: The G Protein-Coupled Receptor in the Genome

 

1. In Silico Identification of Novel G Protein-Coupled Receptors

            Matthew N. Davies, David E. Gloriam, and Darren R. Flower

 

2. Alternative Pre-mRNA Splicing of G Protein-Coupled Receptors

            Ying-Xian Pan, Steven Grinnell, and Gavril W. Pasternak

 

3. Detecting Polymorphisms in G Protein-Coupled Receptor Genes

            Dmitri Proudnikov, Vadim Yuferov, and Mary Jeanne Kreek

 

4. Regulation of the Transcription of G Protein-Coupled Receptor Genes

            Cheol Kyu Hwang, Ping-Yee Law, Li-Na Wei, and Horace H. Loh

 

5. Deciphering the Evolution of G Protein-Coupled Receptors in Vertebrates

            Craig W. Stevens

 

Part II: The Trafficking of G Protein-Coupled Receptors

 

6. A New Approach to Visualize Endogenously Expressed G Protein-Coupled Receptors in Tissues and Living Cells

            Brigitte L Kieffer and Grégory Scherrer

 

7. Post-Translational Regulation of G Protein-Coupled Receptors

            Yu Qiu and Ping-Yee Law

 

8. Discovering G Protein-Coupled Receptor Motifs Mediating Export from the Endoplasmic Reticulum

            Chunmin Dong and Guangyu Wu

 

9. Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface

            Stefano Marullo, Liliana Pardo Lopez, and Lamia Achour

 

10. A Novel Method for Determining the Kinetics of G Protein-Coupled Receptor Plasma Membrane Expression

            Gregory W. Sawyer

 

Part III: The G Protein-Coupled Receptor on the Membrane

 

11. Characterizing the Pharmacology of G Protein-Coupled Receptors in Transfected Cell Lines

            Kathryn A. Seely and Paul L. Prather

 

12. Novel Assay Technologies for the Discovery of G Protein-Coupled Receptor Drugs

            Elisa Alvarez-Curto, Richard J. Ward, and Graeme Milligan

 

13. Discovering Cell-Type-Specific Patterns of G Protein-Coupled Receptor Phosphorylation

            Kok Choi Kong, Andrew B. Tobin, and Adrian J. Butcher

 

14. Quantifying Allosteric Modulation of G Protein-Coupled Receptors

            Frederick J. Ehlert and Hinako Suga

 

15. Experimental Designs for the Study of Receptor-Receptor Interactions

            Dennis Paul

 

Part IV: The Regulation of G Protein-Coupled Receptors

 

16. Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization

            Chris P. Bailey and Eamonn Kelly

 

17. Detecting the Role of Arrestins in G-Protein Coupled Receptor Regulation

            Laura M. Bohn and Patricia H. McDonald

 

18. Characterizing Molecular Mobility and Membrane Interactions of G Protein-Coupled Receptors

            Vladana Vukojević, Yu Ming, and Lars Terenius

 

19. Using RNA Interference to Downregulate G Protein-Coupled Receptors

            Philippe Sarret, Louis Doré-Savard, Pascal Tétreault, Valérie Bégin-Lavallée, Marc-André Dansereau, and Nicolas Beaudet

 

20. Upregulating G Protein-Coupled Receptors with Receptor Antagonists

            Ellen M. Unterwald

Blurb

The goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies.  In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader’s particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors.  Chapters also feature an important section called “Future Directions” which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results.

 

Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors.