Methods for Studying Nucleic Acid/Drug Interactions

ISBN-13: 9781439839737 / Angielski / Twarda / 2011 / 408 str.

Meni Wanunu; Yitzhak Tor

Opis

This book describes emerging, non-conventional methods for probing the interactions of small molecules with nucleic acids as well as modern computational methods for assessment. By highlighting novel methods for nucleic acid/ligand interactions, the text presents drug developing companies with a survey of possible future techniques as well as their drawbacks and advantages with respect to common techniques. In addition, and perhaps more importantly, this book is designed to inspire young scientists to continue and advance these methods into fruition, especially in light of current capabilities for assay miniaturization and enhanced sensitivity using microfluidics and nanomaterials.

Recenzje

"By focusing on a selection of novel and emerging techniques, Wanunu and Tor provide a remarkable overview of biophysical and computational advances in structure-based investigations of the interactions between nucleic acids and small-molecule ligands. An impressive collection of approaches is described: analytical biophysical techniques alongside novel exploitation of chemical and computational tools. Indeed, the smart combination of results obtained by classical methods with state of the art biophysical approaches reveals astonishing insights, thus paving new research avenues in this central area of research."
—Prof. Ada Yonath, Weizmann Institute of Science, 2009 Nobel Laureate in Chemistry

Spis treści

CLASSICAL TECHNIQUES: Using Spectroscopic Techniques to Examine Drug-DNA Interactions. Probing DNA and RNA Interactions with Biogenic and Synthetic Polyamines: Models and Biological Implications. EMERGING TECHNIQUES: Mass Spectrometry-Based Techniques for Studying Nucleic Acid/Small Molecule Interactions.
Real-Time Monitoring of Nucleic Acid Interactions with Biosensor-Surface Plasmon Resonance. Studying Aptamer/Ligand Interactions Using Fluorescence Correlation Spectroscopy. Studying Nucleic Acid - Drug Interactions at the Single Molecule Level Using Optical Tweezers. Fluorescent Nucleoside Analogues for Monitoring RNA–Drug Interactions. Atomic Force Microscopy Investigation of DNA-Drug Interactions. Characterizing RNA-Ligand Interactions Using 2-Dimensional Combinatorial Screening. EPR Spectroscopy for the Study of RNA-Ligand Interactions. Electrochemical Approaches to the Study of DNA-Drug Interactions. Nanopore Ion Microscope for Detecting Nucleic Acid/Drug Interactions. A Primer for Relaxation Kinetic Measurements. DNA-Drug Interactions: A Theoretical Perspective. Computational Studies of RNA Dynamics and RNA-Ligand Interactions.

All chapters include case studies, background and basics, prospects and outlook, conclusions, and references