Metallointercalators: Synthesis and Techniques to Probe Their Interactions with Biomolecules

ISBN-13: 9783709116692 / Angielski / Miękka / 2014 / 427 str.

Janice Aldrich-Wright

Opis

A comprehensive treatment of the characterisation techniques used in investigating inorganic and organic molecules that interact with biomolecules is presented to the reader in a clear fashion. The work consists of two parts: (i) synthetic aspects of metallointercalators along with targeting and improving transport and (ii) the various techniques that are used for probing their interactions, such as; DNA-NMR, PGSE-NMR, DNA ESI-MS, Linear and Circular Dichroism, Fluorescence Spectroscopy, Confocal Microscopy, Viscosity, TGA and dialysis, Microarrays, biological analysis. Chapters are devoted to the synthesis and the techniques used to study the interactions of inorganic complexes with biomolecules. Considerably detailed examples are used to help illustrate the application of these techniques. This book is a useful resource for an array of inorganic and organic advanced undergraduate and graduate courses and for researchers in drug discovery.

Spis treści

-Introduction.-Synthesis:Platinum Intercalating Complexes; Binuclear Platinum Complexes;Binuclear Ruthenium and Platinum Intercalating Complexes; Sequence Selective Intercalating Complexes; Targeting and Improving Transport, Cucurbit[n]uril, Cylcodextrin and Dendrimers.-Techniques to Probe Interactions with Biomolecules:Introduction to NMR; DNA-NMR; PGSE-NMR; DNA ESI-MS; Investigating Binding Interactions using Linear Dichromism and Circular Dichromism; Flourescence Spectroscopy; Confocal Microscopy; Implementation of synchotron X-ray microscopy for investigations of the cellular uptake and cellular metabolism of metallointercalators; Biomolecular Helical Structure; Viscosity, TGA and Dialysis; Microarrays; Cytotoxicity-Cell lines; Antibacterical activity; Animal testing.