Metabonomics: Methods and Protocols

ISBN-13: 9781493923762 / Angielski / Twarda / 2015 / 259 str.

Jacob T. Bjerrum

Opis

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Spis treści

1. Metabonomics - Analytical Techniques and Associated Chemometrics at a Glance

Jacob T. Bjerrum

 

2. Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy

Gwénaëlle Le Gall

 

3. NMR Spectroscopy of Biofluids and Extracts

Gwénaëlle Le Gall

 

4. High-resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue

Guro F. Giskeødegård, Maria D. Cao, and Tone F. Bathen

 

5. Sample Collection and Preparation of Biofluids and Extracts For Liquid Chromatography-Mass Spectrometry

Peiyuan Yin, Lina Zhou, Xinjie Zhao,  and Guowang Xu

 

6. Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts

Xinjie Zhao, Lina Zhou, Peiyuan Yin, and Guowang Xu

 

7. Sample Collection and Preparation of Biofluids and Extracts For Gas-Chromatography-Mass Spectrometry

Abdul-Hamid M. Emwas, Zeyad A. Al-Talla, and Najeh M. Kharbatia

 

8. Gas Chromatography-Mass Spectrometry of Biofluids and Extracts

Abdul-Hamid M. Emwas, Zeyad A. Al-Talla, Yang Yang, and Najeh M. Kharbatia

 9. Capillary Electrophoresis Mass Spectrometry

Masataka Wakayama, Akiyoshi Hirayama, and Tomoyoshi Soga

 

10. Preprocessing of Raw Metabonomics Data

Riyas Vettukattil

 11. Extracting Meaningful Information From Metabonomics Data Using Multivariate Statistics

Max Bylesjö

 12. Combining Metabonomics and Other -omics Data

Mattias Rantalainen

 

13. The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry With Particular Focus on Metabolomics Research

Abdul-Hamid M. Emwas

 

14.  Metabonomics and Drug Development

Pranov Ramana, Erwin Adams, Patrick Augustijns, and Ann Van Schepdael

 

15. Metabonomics and Toxicology

Liang Zhao and Thomas Hartung

 

16. Metabonomics and Diagnostics

Lucy C. Hicks, Simon J.L. Ralphs, and Horace R.T. Williams

 

17. Metabonomics and Systems Biology

Vicky De Preter