Metabolomics: Methods and Protocols

ISBN-13: 9781617376436 / Angielski / Miękka / 2010 / 312 str.

Wolfram Weckwerth

Opis

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.
Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.

Recenzje

From the reviews:

"This book forms part of an extensive and successful series from Methods in Molecular Biology ... . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researcher's needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007)

Spis treści

PART I. Metabolome analysis using gaschromatography coupled to mass spectrometry (GC/MS) 1 Metabolite profiling in blood plasma Oliver Fiehn and Tobias Kind 2 Non-Supervised Construction and Application of Mass Spectral and Retention Time Index Libraries from Time-of-Flight GC-MS Metabolite Profiles Alexander Erban, Nicolas Schauer, Alisdair R. Fernie, Joachim Kopka 3 Metabolomic profiling of natural volatiles: head space trapping - GC/MS Yury M. Tikunov, Francel W.A. Verstappen & Robert D Hall PART II. Metabolomics - Data integration and data mining 4 Integrative profiling of metabolites and proteins: improving pattern recognition and biomarker selection for systems level approaches Katja Morgenthal, Stefanie Wienkoop, Florian Wolschin, and Wolfram Weckwerth 5 Integrating profiling data: using linear correlation to reveal co-regulation of transcript and metabolites Ewa Urbanczyk-Wochniak, Lothar Willmitzer and Alisdair Fernie 6 Visualization and Analysis of Molecular Data Matthias Scholz and Joachim Selbig 7 A gentle guide to the analysis of metabolomic data Ralf Steuer, Katja Morgenthal, Wolfram Weckwerth, and Joachim Selbig PART III. Capillary electrophoresis coupled to mass spectrometry (CE/MS) 8 CE-MS for Metabolomics Tomoyoshi Soga PART IV. Liquid chromatography coupled to mass spectrometry (LC/MS) for metabolomics and structural elucidation 9 Application of LC-MS analysis in Metabolomics: Reversed-Phase Monolithic Capillary Chromatography and Hydrophilic Chromatography coupled to Electrospray Ionization Mass Spectrometry Vladimir V. Tolstikov, Oliver Fiehn, and Nobuo Tanaka PART V. Electrochemical detection of metabolites 10 HPLC-Separations Coupled with Coulometric-Electrode Array Detectors A Unique Approach to Metabolomics Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah-Daouk, and Wayne R. Matson PART VI. Metabolic Fluxes 11 Determination of metabolic flux ratios from 13C-experiments and GC MS data: protocol and principles Annik Nanchen, Tobias Fuhrer and Uwe Sauer 12 Understanding the Roadmap of Metabolism by Pathway Analysis Stefan Schuster, Axel von Kamp and Mikhail Pachkov PART VII. NMR metabolome analysis 13 Revealing the metabolome of animal tissues using 1H NMR spectroscopy Mark R. Viant 14 NMR Metabonomics - Methods for Drug Discovery and Development Karl-Heinz Ott and Nelly Aranibar