Metabolic Engineering of Bacillus » książka
Metabolic Engineering of Bacillus
ISBN-13: 9783639172348 / Angielski / Miękka / 2009 / 144 str.
Microbial cultures usually produce a significant amount of acidic byproducts which can repress cell growth and product synthesis. Metabolic engineering provides a powerful approach to optimize the cellular activities and improve product yields by genetically manipulating specific metabolic pathways. In this study, an inducible PYK (iPYK) mutant of B. subtilis was constructed based on the outcome of metabolic modeling. The results demonstrate that good cell growth rate and low acetate formation can be attained at an appropriate PYK expression level. In Addition, the PYK mutation was explored as an efficient way to improve the production of recombinant protein and valuable metabolites such as folic acid. A combination of PYK mutation and additional mutations in the folic acid synthesis pathway resulted in an 8-fold increase in folic acid production. Recombinant protein was improved two-fold by the PYK mutation due to low acetate formation and longer production time in the pyk mutant.
Microbial cultures usually produce a significantamount of acidic byproducts which can repress cellgrowth and product synthesis. Metabolic engineeringprovides a powerful approach to optimize the cellularactivities and improve product yields by geneticallymanipulating specific metabolic pathways. In thisstudy, an inducible PYK (iPYK) mutant of B. subtiliswas constructed based on the outcome of metabolicmodeling. The results demonstrate that good cellgrowth rate and low acetate formation can be attainedat an appropriate PYK expression level. In Addition,the PYK mutation was explored as an efficient way toimprove the production of recombinant protein andvaluable metabolites such as folic acid. Acombination of PYK mutation and additional mutationsin the folic acid synthesis pathway resulted in an8-fold increase in folic acid production. Recombinantprotein was improved two-fold by the PYK mutation dueto low acetate formation and longer production timein the pyk mutant.
Czytaj nas na:
KrainaKsiazek.PL - Księgarnia Internetowa
