Mesenchymal Stem Cell Assays and Applications

ISBN-13: 9781607619987 / Angielski / Twarda / 2011 / 534 str.

Mohan C. Vemuri; Lucas G. Chase; Mahendra S. Rao

Opis

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Spis treści

Mesenchymal Stem Cell Assays and Applications Editors: Mohan C. Vemuri, Lucas G. Chase and Mahendra S. Rao Table of Contents Preface Contributors Part 1-Introduction 1. Mesenchymal Stem Cell Assays and Applications Mohan C. Vemuri, Lucas G. Chase and Mahendra S. Rao Part 2- Isolation and Expansion of MSCs from Various Sources 2. Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow Patrice Penfornis and Radhika Pochampally 3. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis Patrick Horn, Simone Bork and Wolfgang Wagner 4. Isolation and Growth of Adipose Tissue-Derived Stem Cells Vladimir Zachar, Jeppe Grøndahl Rasmussen and Trine Fink 5. Isolation, Culture and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells Alp Can and Deniz Balci 6. The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions Anita Laitinen, Johanna Nystedt and Saara Laitinen 7. Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture Justin D. Klein and Dario O. Fauza 8. Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells Nina Ilic, Gary Brooke, Patricia Murray, Sarah Barlow, Tony Rossetti, Rebecca Pelekanos, Sonia Hancock and Kerry Atkinson 9. A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells Stan Gronthos, Agnieszka Arthur, P. Mark Bartold and Songtao Shi 10. Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas Karen L. Seeberger, Alana Eshpeter, and Gregory S. Korbutt 11. Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells Ruenn Chai Lai, Andre Choo and Sai Kiang Lim 12. Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells Nance Beyer Nardi and Melissa Camassola 13. Cryopreservation and Revival of Mesenchymal Stromal Cells Mandana Haack-Sørensen and Jens Kastrup 14. Dynamic Expansion Culture for Mesenchymal Stem Cells Hicham Majd, Thomas M. Quinn, Pierre-Jean Wipff, and Boris Hinz 15. Ex-Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers Francisco dos Santos, Pedro Z. Andrade, Gemma Eibes, Cláudia Lobato da Silva and Joaquim M.S. Cabral Part 3- MSC Lineage Differentiation and Analysis 16. Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells Deepak M. Gupta, Nicholas J. Panetta and Michael T. Longaker 17. Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells Ulf Krause, Anja Seckinger and Carl A. Gregory 18. Bioreactor Cultivation of Functional Bone Grafts Warren L. Grayson, Sarindr Bhumiratana, Christopher Cannizzaro and Gordana Vunjak-Novakovic 19. Adipogenic Differentiation of Human Mesenchymal Stem Cells Trine Fink and Vladimir Zachar 20. Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks. Luis A. Solchaga, Kitsie J. Penick and Jean F. Welter 21. Usage of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineering Zhaodi Gong and Laura E. Niklason 22. Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells Katarzyna A. Trzaska and Pranela Rameshwar 23. Hepatic Differentiation of Mesenchymal Stem Cells: in vitro Strategies Sarah Snykers, Joery De Kock, Vanhaecke Tamara and Vera Rogiers 24. Hepatic Transplantation of Mesenchymal Stem Cells in Rodent Animal Models Bruno Christ, Sandra Brückner and Peggy Stock 25. Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells Lindolfo da Silva Meirelles and Dimas Tadeu Covas Part 4- MSC Phenotypic Characterization and Extended Applications 26. Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Osteogenic and Adipogenic Lineages Zheng Yang, Jacqueline Frida Schmitt and Eng Hin Lee 27. Panel Development for Multicolor Flow Cytometry Testing of Proliferation and Immunophenotype in hMSCs Jolene A. Bradford and Scott T. Clarke 28. Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiation Shayne E. Boucher 29. Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins Tzu-Hao Wang, Yun-Shien Lee, Shiaw-Min Hwang 30. Comparison of Microarray and Quantitative Real-Time PCR Methods for Measuring MicroRNA Levels in MSC Cultures Cynthia Camarillo, Mavis Swerdel, and Ronald P. Hart 31. Two Dimensional Gel Electrophoresis Analysis of Mesenchymal Stem Cells Monique Provansal, Christian Jorgensen, Sylvain Lehmann, Stéphane Roche 32. Proteomic Analysis of Human Mesenchymal Stem Cells Guo Li, Chu-yan Chan, Hua Wang and Hsiang-fu Kung 33. Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Differentiation Courtenay Hart, Lucas G. Chase, Mahbod Hajivandi and Brian Agnew 34. BacMam-Mediated Gene Delivery Into Multipotent Mesenchymal Stromal Cells Michael O’Grady, Robert H. Batchelor, Kelly Scheyhing, Christopher W. Kemp, George T. Hanson and Uma Lakshmipathy 35. Cell Surface Engineering of Mesenchymal Stem Cells Debanjan Sarkar, Weian Zhao, Ashish Gupta, Wei Li Loh, Rohit Karnik, Jeffrey M. Karp