Meningococcal Vaccines: Methods and Protocols

ISBN-13: 9780896038011 / Angielski / Twarda / 2001 / 421 str.

Andrew J. Pollard; Martin C. J. Maiden

Opis

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties. Few if any pathogens surpass the meningococcus in the rapidity and sev- ity with which it devastates previously healthy individuals. The challenge of understanding the biology of this fascinating microbe is immense, but few will doubt that successful control of meningococcal meningitis and septicemia will only transpire through the application of a body of extraordinary detailed inf- mation, including key minutiae of its molecular biology. In the first of two companion volumes, Meningococcal Vaccines, the team of experts gathered by Andy Pollard and Martin Maiden converge to p- vide an impressive accumulation of molecular tools with which to lay bare the secrets of the meningococcus. The results of this ambitious and welcome v- ume represent an exciting and much needed resource for all of us in the field.

Recenzje

"This volume is an impressive collection of the latest techniques in the development, evaluation and application of meningococcal vaccines. The easy-to-follow protocols each preceded by a brief introduction into the topic were provided by leading scientists in the field. Andrew Pollard and Martin Maiden did a great job in assembling these protocols into one volume - a work we all in the meningococcal field have waited for." - International Journal of Medical Microbiology.

"The strength of this text is that it provides a key reference source, enabling researchers in the field to avoid reinventing the wheel. The text also provides the basis for the development of new ideas. Each chapter takes a step-by-step approach with clearly set out notes highlighting the problem areas. All the chapters have comprehensive bibliographies, and are well structured. The text is easily accessible and is recommended to those who are seeking to critically appraise the field of meningococcal vaccines." - Epidemiology and Infection

"This book and its accompanying volume are well produced, easy to read and well edited. Andrew Pollard and Martin Maiden are to be congratulated in bringing together in these two volumes an enormous amount of information about this very important topical infection." -Molecular Biotechnology

Spis treści

Meningococcal Vaccines and Vaccine Developments Ian M. Feavers Immune Response and Host-Pathogen Interactions Andrew J. Pollard and David Goldblatt Purification of Capsular Polysaccharide Qingling Yang and Harold Jennings Protein-Polysaccharide Conjugation Zhongwu Guo and Harold Jennings N-Propionylation Zhongwu Guo and Harold Jennings Outer Membrane Protein Purification, armen Arigita, Wim Jiskoot, Matthijs R. Graaf, and Gideon F. A. Kersten Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, and Einar Rosenqvist Methods for Manipulation of Transferrin-Binding Proteins Leanne M. DeWinter and Anthony B. Schryvers Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins Richard F. Collins and Jeremy P. Derrick Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics Janet Suker and Bambos M. Charalambous Construction of porA Mutants Peter van der Ley and Loek van Alphen Construction of LPS Mutants Peter van der Ley and Liana Steeghs Recombinant Proteins in Vaccine Development Myron Christodoulides, Keith A. Jolley, and John E. Heckels Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries Paul J. Brett, Ian M. Feavers, and Bambos M. Charalambous Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity Alexei A. Delvig, John H. Robinson, and Lee Wetzler Early Experiences with Nasal Vaccines against Meningococcal Disease Johan Holst and Bjørn Haneberg Animal Models for Meningococcal Disease Andrew R. Gorringe, Karen M.Reddin, Pierre Voet, and Jan T. Poolman Determination of Antibody Responses to Meningococcal Antigens by ELISA Einar Rosenqvist, Helena Käyhty, and Andrew J. Pollard Immunoblot Analysis of Sera from Patients and Vaccinees Elisabeth Wedege Serogroup B and C Serum Bactericidal Assays Ray Borrow and George M. Carlone A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers Dan M. Granoff and John J. Donnelly Whole-Blood Model Catherine A. Ison Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry Terje E. Michaelsen and Audun Aase T-Cell Responses Against Meningococcal Antigens Lisbeth Meyer Næss, Fredrik Oftung, Audun Aase, Terje E. Michaelsen and Andrew J. Pollard CD4 T-Cell Epitope Mapping Alexei A. Delvig and John H. Robinson Epitope Mapping John E. Heckels and Myron Christodoulides Meningococcal Vaccine Trials Paddy Farrington and Elizabeth Miller The Introduction of Group C Conjugate Meningococcal Vaccine into the UK David M. Salisbury

Blurb

The development of a comprehensive vaccine against Neisseria meningitidis, the causative agent of meningococcal disease, has remained elusive because of bacterial diversity and immunologic evasion. In Meningococcal Vaccines: Methods and Protocols, Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. They also present as a test case the recent implementation of a new meningococcal vaccine in the United Kingdom. A companion volume, Meningococcal Disease: Methods and Protocols, contains detailed methods for diagnostic microbiology, bacterial characterization, epidemiology, host-pathogen interactions, and clinical studies.
Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus and to develop, evaluate, and implement successful new meningococcal vaccines.