Membrane Transporters: Methods and Protocols

ISBN-13: 9781617373596 / Angielski / Miękka / 2010 / 372 str.

Qing Yan

Opis

Studies of membrane transporters have had great impact on our und- standing human diseases and the design of effective drugs. About 30% of current clinically marketed drugs are targeting membrane transporters or channels. Membrane Transporters: Methods and Protocols provides various practical methodologies for the ongoing research on membrane transporters. To provide readers the most up-to-date information, several emerging fields and methodologies are embraced in this book, including pharmacogenomics, bioin-formatics, and microarray technology. Pharmacogenomics studies of membrane transporters are useful in drug discovery and in predicting drug responses in the clinic. In this volume, the current status of pharma- genomics studies of transporters is reviewed and research methodologies in this field are described. Transporter classification is important in studying the structure and function of membrane transporters and has thus triggered intensive interest in recent years. Membrane Transporters: Methods and Protocols provides a systematic classification of all transmembrane transport proteins found in living org- isms on Earth. This classification system will be helpful for further studies on various aspects of membrane transporters, especially for such large-scale gene expression studies as those employing microarray technologies. Bioinformatics is frequently used in transporter studies and has become indispensable for all kinds of research methods. Commonly used bio- formatics methods, such as databases and tools for sequence analysis and - tif studies, are explained in order to facilitate membrane transporters research. Because of heterogeneous sources and tremendous amounts of data, data in- gration has become one of the most important issues in transporter studies.

Recenzje

"The book is a useful reference to have for bench scientists. The protocols provide a good starting point for someone seeking technical advice for various procedures used to study transport."-Doody's Health Sciences Book Review Journal

"...a valuable laboratory or office protocols book that combines, distils and updates traditional and novel bench and in silico techniques for the study of membrane transporters." - Clinical and Experimental Pharmacology and Physiology

Spis treści

Pharmacogenomics of Membrane Transporters: An Overview Qing Yan The IUBMB-Endorsed Transporter Classification System Wolfgang Busch and Milton H. Saier, Jr. Bioinformatics and Data Integration in Membrane Transporter Studies Qing Yan DNA Microarrays to Analyze Gene Expression in Animals With Altered Transporter Expression Gary W. Miller Microarray Data Analysis in Studies of Membrane Transporters Donglei Hu Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions Okkyung Rho and Gary W. Miller Site-Directed Mutagenesis in the Study of Membrane Transporters Renae M. Ryan, Ann D. Mitrovic, and Robert J. Vandenberg Fluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug Transporter Frances J. Sharom, Paula L. Russell, Qin Qu, and Peihua Lu Reconstitution of Membrane Transporters Frances J. Sharom and Paul D. W. Eckford Equilibrium Binding and Transport Studies Ana M. Ojeda, Dawn T. Bravo, Terry L. Hart, and Stanley M. Parsons Electrophysiological Studies of Ion Channels and Therapeutic Potential with Adenovirus-Mediated Gene Transfer Jihong Qu, Thai V. Pham, and Maria N. Obreztchikova Antibody Production and Immunocytochemical Localization of Amino Acid Transporters David V. Pow, Robert Sullivan, and Heather Scott Xenopus laevis Oocytes Stefan Bröer Measurement of Intracellular pH Frederick B. Loiselle and Joseph R. Casey Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains Brian H. Shilton Membrane Protein NMR Studies Stanley J. Opella Analysis of Soluble Sugar Permease Domains by Solution NMR Thomas A. Wilkinson and Yuan Chen Molecular Modeling and Molecular Dynamics Simulationsof Membrane Transporter Proteins Donard S. Dwyer Imaging Plasma Membrane Proteins by Confocal Microscopy Stella W. Y. Tsang and Peter M. Haney Index

Blurb

Membrane transporters and channels play key roles in drug absorption and distribution, influence drug efficacy and toxicity, and are the target of about 30% of currently marketed drugs. In Membrane Transporters: Methods and Protocols, a panel of recognized basic and clinical investigators-many world-famous-describe their proven, cutting-edge methodologies for studying these important proteins. Treated in step-by-step detail, these techniques take advantage of all the latest developments in biomedical research, including pharmacogenomics, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics analysis, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small-angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microscopy, are also described, along with several methods that may help in the development of novel therapeutics. Each technique contains hands-on instructions, many notes on avoiding the problems that one may encounter, and suggestions for variant procedures.
Comprehensive and highly practical, Membrane Transporters: Methods and Protocols offers investigators working in biopharmaceutical science, drug discovery, and drug delivery both an array of powerful tools for work on membrane transporters and an essential guide to the state-of-the-art in the membrane transporter field today.