Membrane Lipids: Methods and Protocols

ISBN-13: 9781071618455 / Angielski / Miękka / 2021 / 300 str.

Charles G. Cranfield

Opis

This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.

Spis treści

1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles

            Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang

 

2. Preparing Ion Channel Switch Membrane-Based Biosensors

            Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela

 

3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer

            Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt

 

4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes

            Tadas Penkauskas, Filipas Ambrulevičius, and Gintaras Valinčius

 

5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes

            Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster

 

6. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)

            Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster

 

7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

            Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield

 

8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

            Kan Shoji

 

9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations

            Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes

 

10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations

            Robby Manrique

 

11. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins

            Amanda Buyan and Ben Corry

 

12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling

            Kathleen Wood

 

13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation

            Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt

 

14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

            Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt

 

15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers

            Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson

 

16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs)

            Kinga Burdach, Damian Dziubak, and Slawomir Sek

 

17. Manipulation of Lipid Membranes with Thermal Stimuli

            Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen

 

18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid β Oligomers by Atomic Force Microscopy

            Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta

 

19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains

            Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté

 

20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR

            Elvis Pandzic, Renee Whan, and Alex Macmillan

 

21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides

            Srikanth Dumpati and Debarun Dutta

 

22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi

            Maria Makarova and Dylan M. Owen