Mass Spectrometry of Glycoproteins: Methods and Protocols

ISBN-13: 9781071612408 / Angielski / Twarda / 2021 / 401 str.

Arnaud Delobel

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Glycosylation of Therapeutic Proteins: A Critical Quality Attribute

Arnaud Delobel

2. Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry

Dan Bach Kristensen, Trine Meiborg Sloth, Martin Ørgaard, and Pernille Foged Jensen

3. Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Estela Giménez, Montserrat Mancera-Arteu, Fernando Benavente, and Victoria Sanz-Nebot

4. Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MS

Yosra Helali, Shilpee Sharma, Marie Vandeput, Dansala Welba, Pierre Van Antwerpen, Arnaud Marchant, and Cédric Delporte 

5. Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass Spectrometry

Elsa Wagner-Rousset, Olivier Colas, Stéphane Chenu, Yannis-Nicolas François, Davy Guillarme, Sarah Cianferani, Yury O. Tsybin, Jonathan Sjögren, Arnaud Delobel, and Alain Beck

6. Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS

Valentina D’Atri and Davy Guillarme

7. Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis – Mass Spectrometry Coupling (CE-MS)

Josiane Saadé, Michael Biacchi, Jérémie Giorgetti, Antony Lechner, Alain Beck, Emmanuelle Leize-Wagner, and Yannis-Nicolas François

8. Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography

Abel Bermudez and Sharon J. Pitteri

9. In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC –SPE and LC-MS/MS

Myung Jin Oh, Youngsuk Seo, Unyong Kim, and Hyun Joo An

10. Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE

Kevin Canis, Estelle Garénaux, and Jean-François Boe

11. Mapping O-glycosylation Sites using OpeRATOR and LC-MS

Maria Nordgren, Andreas Nägeli, Helén Nyhlén, and Jonathan Sjögren

12. Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional Dissociation

Noritaka Hashii and Junya Suzuki

13. Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent Anthranilamide

Claire I. Butré, Eric Largy, and Arnaud Delobel

14. Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS Labeling

Rosie Upton, James Duffy, Sam Clawson, and David Firth

15. Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass Detection

Ximo Zhang, Vithiya Vimalraj, and Mitul Patel

16. Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals 

Sonja Schneider, Edgar Naegele, and Sonja Krieger

17. Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent Anthranilamide

Claire I. Butré, Eric Largy, Fabrice Cantais, and Arnaud Delobel

18. Linkage Analysis of Oligo- and Polysaccharides: A Tutorial

Ian Black, Christian Heiss, Russell W. Carlson, and Parastoo Azadi

19. Use of Exoglycosidases for the Structural Characterization of Glycans

Beth McLeod, Paula Magnelli, and Xiaofeng Shi

20. Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS)

Byeong Gwan Cho, Alireza Banazadeh, Wenjing Peng, Jingfu Zhao, Mona Goli, Sakshi Gautam, Ahmed Hussein, and Yehia Mechref

21. Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded Tissues

Connor A. West, Xiaowei Lu, Grace Grimsley, Kim Norris-Caneda, Anand S.  Mehta, Peggi M. Angel, and Richard R. Drake

22. Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS

Jesús S. Aguilar Díaz de león and Chad R. Borges

23. Array-Based N-Glycan Profiling of Cells in Culture

Peggi M. Angel, Anand S. Mehta, and Richard R. Drake

24. Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multi-Angle Light Scattering

Kathryn Hastie, Vamseedhar Rayaprolu, and Erica Ollmann Saphire

25. Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment

Allison Derenne, Kheiro-Mouna Derfoufi, Ben Cowper, Cédric Delporte, Claire I. Butré, and Erik Goormaghtigh

26. Deploying Mass Spectrometric Data Analysis in the Amazon AWS Cloud Computing Environment

Jonathan E. Katz

Subject Index List…

Blurb

This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.