Mass Spectrometry Imaging of Small Molecules

ISBN-13: 9781493913565 / Angielski / Twarda / 2014 / 213 str.

Lin He

Opis

Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules Methods and Protocols, experts in the MSI field present techniques for 2D and 3D visualization and quantification of a wide array of small molecular species present in biologically relevant samples. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Mass Spectrometry Imaging of Small Molecules Methods and Protocols aims to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.

Recenzje

"The purpose is to detail the rapidly maturing methods of metabolic imaging and to minimize technical challenges in adapting new technological platforms in biological research. These are quite worthy objectives that the book meets. The intended audience is life science researchers. The book also will be quite useful for students and scientists interested in learning more about mass spectrometry imaging of small molecules." (Omer Iqbal, Doody's Book Reviews, September, 2015)

Spis treści

1. Current Status and Future Prospects of Mass Spectrometry Imaging of Small Molecules

Victoria L. Brown and Lin He

 

2. Sample Preparation for 3D SIMS Chemical Imaging of Cells

Nicholas Winograd and Anna Bloom

 

3. TOF-SIMS Imaging of Lipids on Rat Brain Sections

David Touboul and Alain Brunelle

 

4. MALDI-MS Assisted Molecular Imaging of Metabolites in Legume Plants

Erin Gemperline and Lingjun Li

 

5. MALDI Mass Spectrometry Imaging of Lipids and Primary Metabolites on Rat Brain Sections

David Touboul and Alain Brunelle

 

6. Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System

Andrew R. Korte, Gargey B. Yagnik, Adam D. Feenstra, and Young Jin Lee

 

7. DESI Imaging of Small Molecules in Biological Tissues

Elaine C. Cabral and Demian R. Ifa

 

8. Desorption Electrospray Ionization Imaging of Small Organics on Mineral Surfaces

Rachel V. Bennett and Facundo M. Fernández

 

9. Imaging of Plant Materials Using Indirect Desorption Electrospray Ionization Mass Spectrometry

Christian Janfelt

 

10. Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Ingela Lanekoff and Julia Laskin

 

11. Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for Molecular Imaging of Small Molecules on Tissues

Min-Zong Huang, Siou-Sian

Jhang, and Jentaie Shiea

 

12. Automated Cell-By-Cell Tissue Imaging and Single Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry

Hang Li, Brian K. Smith, Bindesh Shrestha, Lászlo Márk, and Akos Vertes

 

13. Laser Ablation Sample Transfer for Mass Spectrometry Imaging

Sung-Gun Park and Kermit K. Murray

 

14. Nanostructure-Based Mass Spectrometry: High Sensitivity, Enzyme Activity Analysis and Metabolite Imaging

Michael E. Kurczy, Trent R. Northen, Sunia A. Trauger, and Gary Siuzdak

 

15. Nanostructure-Initiator Mass Spectrometry (NIMS) for Molecular Mapping of Animal Tissues

Tara N. Moening, Victoria L. Brown, and Lin He

 

16. Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging

Michihiko Waki, Eiji Sugiyama, Takeshi Kondo, Keigo Sano, and Mitsutoshi Setou

 

17. Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules

Victoria L. Brown, Qiang Liu, and Lin He

 

18. Laser Desorption Postionization Mass Spectrometry Imaging of Biological Targets

Artem Akhmetov, Chhavi Bhardwaj, and Luke Hanley

 

19. Data Processing and Analysis for Mass Spectrometry Imaging

Jiangjian Liu, Xingchuang Xiong, and Zheng Ouyang.

Blurb

Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules, experts in the MSI field present techniques for 2D and 3D visualization and quantification of a wide array of small molecular species present in biologically relevant samples. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification, and from data collection to visualization of small molecule mapping in complex samples. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Mass Spectrometry Imaging of Small Molecules aims to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.