Map Kinase Signaling Protocols

ISBN-13: 9781607617945 / Angielski / Twarda / 2010 / 529 str.

Rony Seger

Opis

Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases. Content Level Professional/practitioner

Spis treści

Part I: Activation and Function of Components of the MAP Kinase Signaling Cascades 1. The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions Yonat Keshet and Rony Seger 2. Determination of ERK Activity: Anti-Phospho-ERK Antibodies and In vitro Phosphorylation Shiri Procaccia, Sarah Kraus, and Rony Seger 3. Activation of SAPK/JNKs In vitro Deborah N. Chadee and John M. Kyriakis 4. Activation of p38 and Determination of Its Activity Huamin Zhou, Jianming Chen, and Jiahuai Han 5. Activity Assays for Extracellular Signal-Regulated Kinase 5 Kazuhiro Nakamura and Gary L. Johnson 6. Use of Inhibitors in the Study of MAP Kinases Kimberly Burkhard and Paul Shapiro Part II: Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals 7. MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration Gabi Tarcic and Yosef Yarden 8. Activation of Ras and Rho GTPases and MAP Kinases by G Protein-Coupled Receptors Mario Chiariello, Jose P. Vaqué, Piero Crespo, and J. Silvio Gutkind 9. Regulation of MAP Kinase Signaling by Calcium Colin D. White and David B. Sacks 10. Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates Hui Zheng, Adnan Al-Ayoubi, and Scott T. Eblen 11. ERK-MAP Kinase Signaling in the Cytoplasm Michelle C. Mendoza, Ekrem Emrah Er, and John Blenis 12. Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases Marzia Indrigo, Alessandro Papale, Daniel Orellana and Riccardo Brambilla Part III: Structure-Function Relationships and Localization of MAP Kinases 13. Structural Studies of MAP Kinase Cascade Components Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, and Tianjun Zhou 14. Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry Kevin M. Sours and Natalie G. Ahn 15. A ‘Molecular Evolution’ Approachfor Isolation of Intrinsically Active (MEK-Independent) MAP Kinases Vered Levin-Salomon, Oded Livnah, and David Engelberg 16. Reconstitution of the Nuclear Transport of the MAP Kinase ERK2 Arif Jivan, Aarati Ranganathan, and Melanie H. Cobb 17. Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2 Matilde Marchi, Riccardo Parra, Mario Costa, and Gian Michele Ratto Part IV: Studies on the Regulation of MAP Kinase Cascades 18. Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases in vitro and in Cell Systems Céline Tárrega, Caroline Nunes-Xavier, Rocío Cejudo-Marín, Jorge Martín-Pérez, and Rafael Pulido 19. Proteomic Analysis of Scaffold Proteins in the ERK Cascade Melissa M. McKay and Deborah K. Morrison 20. Analysis of ERKs’ Dimerization by Electrophoresis Adán Pinto and Piero Crespo 21. MAP Kinase - SUMO Pathway Interactions Shen-Hsi Yang and Andrew D. Sharrocks 22. Computational Modelling of Kinase Signalling Cascades David Gilbert, Monika Heiner, and Rainer Breitling Part V: Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases 23. Analysis of MAP Kinase Activity in Yeast Elaine A. Elion and Rupam Sahoo 24. Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining Aharon Helman and Ze'ev Paroush 25. Studying MAP Kinase Pathways During Early Development of Xenopus laevis Aviad Keren and Eyal Bengal 26. Deciphering Signaling Pathways In vivo: The Ras/Raf/Mek/Erk Cascade Gergana Galabova-Kovacs and Manuela Baccarini 27. Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes William E. Tidyman and Katherine A. Rauen Part VI: Study of MAP Kinases in Specific Physiological Systems 28. Implication of the ERK Pathway on the Post Transcriptional Regulation of VEGF mRNA Stability Khadija Essafi-Benkhadir, Jacques Pouysségur, and Gilles Pagès 29. Studies on MAP Kinase Signaling in the Immune System Hongbo Chi and

Blurb

Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases.